Alignments and conserved secondary structures for cis-acting 2C replication elements conserved within HRV-B and HEV. A) Multiple sequence alignment across all considered genomes that shows consensus secondary RNA structure (in dot bracket format, see first row); sequences are colour-coded according to RNA structure conservation; the sequence conservation profile for each group is shown in grey bars beneath the alignments. B) Secondary structure of the conserved cre 2C, colour-coded according to the different types of base pairs in the corresponding alignment columns. The more different the types of base pairs existing for two pairing alignment columns, the more evolutionary conservation of the structure (cp. compensatory and consistent mutations).