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Table 1 Resting NK signature derived from Affymetrix and spotted microarray platform.

From: Genome wide transcriptional analysis of resting and IL2 activated human natural killer cells: gene expression signatures indicative of novel molecular signaling pathways

   Median fold change (Range)
Gene ontogeny Gene symbols Spotted Affymetrix
Cell cycle regulators BTG1, JAG1, MATK, TGFBR3, TINF2, TMEPAI, NEK1, CCND2, CCNC, BIN1, HEXIM1, PPP2R5A, CARF 2.59 (2.01–5.06) 3.76 (2.87–7.05)
Cytotoxicity CRTAM, CTSW, DPP8, FASLG, FNBP1, GNLY, GZMA, GZMH, HCST, LITAF, PRF1, TNFRSF1B, TNFSF14, NEK1, CASP10, STK17B, CTSC, PRSS23 4.48 (2.36–12.76) 4.43 (2.8–19. 42)
Cytokines CCL5, CX3CR1, IL18R1, IL18RAP, IL21R, PPBP(CXCL7) 7.12 (2.9–17.56) 5.93 (2.86–29.7)
Inflammatory response C3AR1, PDE7A, LY75, RORA, AOAH, ARF6, C1orf38, CLEC2B, IRF1 3.12 (2.20–6.56) 5.06 (2.83–11.01)
Regulators of immune response PTPN4(negative regulators), PPP2R2B, SLA2, ID2, KLF11, ZFHX1B 3.18 (2.36–8.6) 9.92 (2.84–11.39)
Secretory pathway and protein maturation GALNT11, GNPTAB, RAMP1, SEC22L3, SLC38A1, TES, TPST2, WDFY1, GALNT7, DNAJC3, SLC35E1, ZNF12 3.88 (2.90–9.01) 4.34 (3.00–4.96)
Transcription factors and accessory regulators BHLHB2, EOMES, KBTBD2, MAF, NFAT5, THRAP3, TXK, ZBTB11, ZNF267, ZNF394, ZNF12, HCF2, MLLT10, MLL3, SSRPN, TAF1, MLR1, ZNF614, EIF2C4, EIF1AY, EIF2C2, 3.55 (2.58–7.44) 3.71 (2.88–4.9)
Regulation of GTPase activity CENTD1, PLEK, PREX1, RAB7L1, RASGRP1, RGS18, RGS3, RIN3, PCTK2, RAB27B 4.16 (2.91–5.67) 4.43 (3.64–6.8)
Proliferative GFI1, SMAD5, ZBTB16, AKT3, SMC5L1, RNF139 2.90 (2.90–22.16) 3.02 (3.02–5.3)
Cytoskeleton orgnisation DOCK5, DOCK8, DOK2, MACF1, MYOM2, PHLDB2 3.41 (2.73–6.21) 3.51 (2.94–8.50)
Metabolism and nucleotide biosynthesis DCY7, ATP6AP2, CHST12, CLIC3, GFOD1, PARP8, TKTL1, YPEL1, ENPP4, DHRS7, BPGM 2.92 (2.25–19.24) 4.98 (2.84–9.55)
Miscellaneous SBK1, PLAC8, PLCL2, RNF125, SPAG9, MAPK1, C10orf137, C10orf18, C1orf63, C20orf3, FLJ13611, FLJ39739, GALNT10, ZBTB11, BTBD9, C14orf43, BRWD1, FAM62B, BCAP29, C18orf8, LOC285628, KLHL21, KIAA0907, KIAA1450, MOBKL2A, TSPYL1, TRIM39, ULK2, ULK4, WAC, YTHDC2, ZSWIM6 4.16 (2.42–10.13) 4.23 (2.94–8.17)
Cell adhesion and cell surface ITGAM, ITGB2, ADAM8, FGR, CD300A(Irp60), CD3Z, CD96, FCER1G, CD94 (KLRD1), CD314 (KLRK1), LAIR2, ADD3, CD244, SPN(CD43), KLRF1 3.36 (2.36–8.9) 3.90 (2.4–21.53)
  1. Resting NK signature of genes showing 2 fold higher expression levels on two microarray platforms. A common set of 164 genes were more than 2 fold higher expressed in resting NK cells compared to 1) the lymphoid RNA standard, tonsillar cells and resting CD8+ T cells on spotted microarrays and 2) the lymphoid RNA standard, universal RNA standard (Stratagene Inc, CA) and resting CD8+ T cells on GeneChipU133plus 2® (Affymetrix Inc, CA).