Multiple sequence alignment of 5' end of coding and non-coding regions of HSC70 family. The arrows and red characters represent the highly polymorphic sites for the qRT-PCR primers. The bold characters represent the HSC70B gene and the DNA template for the dsRNA of HSC70B. The asterisks denote the conserved sequences among the HSC70 gene family. Accession numbers of the nucleotide and amino acid sequences used: An. gambiae: HSC70A, ENSANGG00000019768; HSC70B, ENSANGG00000017398; HSC70C, ENSANGG00000016503; HSC70D, ENSANGG00000010404; HSC70E, ENSANGG00000012804.