Anopheles gambiae heat shock protein cognate 70B impedes o'nyong-nyong virus replication

BMC Genomics

Table 1 The primer list of qRT-PCR, RT-PCR, and dsRNAs template

Gene ID (GenBank Accession No.)	Primer sequence (5' to 3')	Product size (bp)	Amplification efficiency^c (R^d)
^aqRpS4 (AJ283756)	Forward: GCTGCCGCTGGTGATCTT Reverse: TCGTCACCTCGCTGTTGGT	65	0.90 (0.99)
qHSC70B (AL930714)	Forward: GCGATCCAGGCCGACAT Reverse: TCTTTGGCTTGCCCTCGAT	64	0.97 (0.99)
EF-1α (XM308429.1)	Forward: CGAGAAGGAAGCTCAGGAGA Reverse: ACGACGACACCTCCTTCTTG	404
agglutinin (XM311465.2)	Forward: CGGGCGAAACTTACTACAGC Reverse: CGATGGCTACGTTACGGAAT	435
HSC70B (AL930714)	Forward: GTGAACGAGGCCGAGAAGTA Reverse: TAGTCGACCTCCTCGATGGT	410
RpS7 (XM_314557.2)	Forward: ATCGCTATGGTGTTCGGTTC Reverse: GCTGCAAACTTCGGCTATTC	627
E1 (AF079456.1)	Forward: AGAGCCCACACAGCTTCAGT Reverse: ACCGGGTTTGTTGCTATCTG	431
NS1 (AF079456.1)	Forward: CTTCCTGATGTGCAAGACGA Reverse: ACGACCACAGGCTTGGTATC	440
^bdsEF-1α (XM308429.1)	Forward: TAATACGACTCACTATAGGCG AGAAGGAAGCTCAGGAGA Reverse: TAATACGACTCACTATAGGAC GACGACACCTCCTTCTTG	442
ds-agglutinin (XM311465.2)	Forward: TAATACGACTCACTATAGGCG GGCGAAACTTACTACAGC Reverse: TAATACGACTCACTATAGGCG ATGGCTACGTTACGGAAT	473
dsHSC70B (AL930714)	Forward: TAATACGACTCACTATAGGTT ACGGTGCGAGCAGAAAAT Reverse: TAATACGACTCACTATAGGCA CGTTCAGTCCCGAGATGT	652
dsβ-gal (NC000913.2)	Forward: TAATACGACTCACTATAGGGG TCGCCAGCGGCACCGCGCCTTTC Reverse: TAATACGACTCACTATAGGGC CGGTAGCCAGCGCGGATCATCGG	545

^aq represent the primer pairs for quantitative RT-PCR, ^bds represents the primer pairs of the templates for dsRNA includes T7 promoter for in vitro transcription. ^cAmplification efficiencies were calculated from the slope of standard curves as E = 10^[-1/slope]-1. 100% PCR efficiency corresponds to an amplification efficiency of 1(Applied Biosystems Application Note); ^dRegression coefficient of linear standard curve.

ISSN: 1471-2164