Table 1 The primer list of qRT-PCR, RT-PCR, and dsRNAs template
From: Anopheles gambiae heat shock protein cognate 70B impedes o'nyong-nyong virus replication
| Gene ID (GenBank Accession No.) | Primer sequence (5' to 3') | Product size (bp) | Amplification efficiencyc (Rd) |
|---|---|---|---|
| aqRpS4 (AJ283756) |
Forward: GCTGCCGCTGGTGATCTT Reverse: TCGTCACCTCGCTGTTGGT | 65 | 0.90 (0.99) |
| qHSC70B (AL930714) |
Forward: GCGATCCAGGCCGACAT Reverse: TCTTTGGCTTGCCCTCGAT | 64 | 0.97 (0.99) |
| EF-1α (XM308429.1) |
Forward: CGAGAAGGAAGCTCAGGAGA Reverse: ACGACGACACCTCCTTCTTG | 404 | |
| agglutinin (XM311465.2) |
Forward: CGGGCGAAACTTACTACAGC Reverse: CGATGGCTACGTTACGGAAT | 435 | |
| HSC70B (AL930714) |
Forward: GTGAACGAGGCCGAGAAGTA Reverse: TAGTCGACCTCCTCGATGGT | 410 | |
| RpS7 (XM_314557.2) |
Forward: ATCGCTATGGTGTTCGGTTC Reverse: GCTGCAAACTTCGGCTATTC | 627 | |
| E1 (AF079456.1) |
Forward: AGAGCCCACACAGCTTCAGT Reverse: ACCGGGTTTGTTGCTATCTG | 431 | |
| NS1 (AF079456.1) |
Forward: CTTCCTGATGTGCAAGACGA Reverse: ACGACCACAGGCTTGGTATC | 440 | |
| bdsEF-1α (XM308429.1) |
Forward: TAATACGACTCACTATAGGCG AGAAGGAAGCTCAGGAGA Reverse: TAATACGACTCACTATAGGAC GACGACACCTCCTTCTTG | 442 | |
| ds-agglutinin (XM311465.2) |
Forward: TAATACGACTCACTATAGGCG GGCGAAACTTACTACAGC Reverse: TAATACGACTCACTATAGGCG ATGGCTACGTTACGGAAT | 473 | |
| dsHSC70B (AL930714) |
Forward: TAATACGACTCACTATAGGTT ACGGTGCGAGCAGAAAAT Reverse: TAATACGACTCACTATAGGCA CGTTCAGTCCCGAGATGT | 652 | |
| dsβ-gal (NC000913.2) |
Forward: TAATACGACTCACTATAGGGG TCGCCAGCGGCACCGCGCCTTTC Reverse: TAATACGACTCACTATAGGGC CGGTAGCCAGCGCGGATCATCGG | 545 |