Expression of SpREJ transcripts during embryogenesis. Transcripts of SpREJ1 and 6 could not be detected in embryos. Transcript copy numbers per 100 ng of starting total RNA at each developmental stage for 8 SpREJs were first calculated. Transcript copy numbers were calculated from a standard curve generated in the same run and were normalized to the level of ubiquitin (SpUbi) transcripts at each developmental time. Final numbers are the averages of three replicas. There are about 50,000 transcripts per 100 ng total RNA of SpREJ8 at 22 hours of development. The standard error of each data point is shown (n = 3). Horizontal axes are hours of development at 16°C.