Figure 4

Expression Analysis of MADS-box genes in rice. a) Hierarchical cluster display of expression profile for thirty seven MADS-box genes showing high level expression in rice. (Color bar at the base represents log₂ expression values, thereby green color representing low level expression, black shows medium level expression and red signifies high level expression). b) Hierarchical cluster display of expression profile for thirty two MADS-box genes showing low level expression in rice. (Color bar at the base represents log₂ expression values, thereby blue color representing low level expression, yellow shows medium level expression and red signifies high level expression). Developmental stages used for expression profiling are mentioned on top of each column. Panicle and seed stages have been listed in the temporal order of development. On the left side of expression map, cluster dendrogram is shown. On the right side, eight groups have been made for the genes showing discrete expression patterns (Figure 5). Symbol * represents accumulative expression profiles of duplicated genes, OsMADS81, 82, 85 and 99. Genes belonging to the different groups have been marked by a symbol (m, MIKC^c; m*, MIKC*; α, Mα; β, Mβ and γ, Mγ). c) Results of QPCR for OsMADS78 and 79. Expression profiles of OsMADS78 and 79 using QPCR. X-axis represents the developmental stages as given in Table 2. Y-axis represents relative expression values obtained after normalizing against maximum expression value. Error bars show the standard error for two biological replicates performed.