Figure 3

Effects of reference genes used for normalization: Relative expression of NRG1, HMMR, PRIM1, and IRAK1 for all stages of HCV-induced HCC. QPCR data were normalized to RPL41 and SFRS4 (shown in pink), to GAPDH (yellow), to ACTB (light blue), to RPS20 (green), and to TBP (brown). Microarray data are shown in dark blue. Fold-changes are indicated on the y-axis. Disease stages as in Figure 2. The table shows p-values for the change in gene expression from high-grade dysplasia to very early HCC for NRG1, HMMR, PRIM1, and IRAK1 (rows) when normalized to the genes indicated above (columns). Significant (p ≤ 0.5) up-regulation between these stages is indicated in red, down-regulation in green.