Figure 5
Induction of the SP212 mRNA in adult flies after infection with different microorganisms. Northern blot analysis of total RNA extracted from adult flies at different times (3, 24 and 48 hours) after challenging by pricking with a needle dipped into 109 cells/ml cultures of either Gram+ (S.aureus) or Gram- (E.coli) bacteria or fungi (A.fumigattus). Note that asceptic pricking by itself triggers the induction of the SP212, albeit at a lower level.