Table 3 Primers used for probes, southern hybridization and PCR
Sequence (5'-3') | Position | Comments | |
|---|---|---|---|
UBA 540F | |||
UBA TmR | TCTTCTTCCAAATGACGACCCC | Exon 5 | cDNA amplification Sasa-UBA |
TAP2.550F | GCGGGACACCGTCAGGGCAGT | Exon 5 | cDNA amplification Sasa-TAP2a/b |
TAP2.850R | CGGCCCCACCAGAGCAGTCAG | Exon 8 | cDNA amplification Sasa-TAP2a/b |
PSMB8.50F | GACTTCGTGGGCAGATTCTT | Exon 2 | cDNA amplification Sasa-PSMB8 |
PSMB8.350R | TCAGCAGCACTACCAGACAT | Exon 3 | cDNA amplification Sasa-PSMB8 |
PSMB9 b4.590F | GAGGACTGCCAACAGTTTGTT | Exon 4 | cDNA clone (2B4) amplification Sasa-PSMB9 |
PSMB9 b4.876R | ATATCATGCTGGCACAATGTT | 3' UTR | cDNA clone (2B4) amplification Sasa-PSMB9 |
UBAlead1.F | CTGGGAATAGGCCTTCTACAT | Exon 1 | Sasa-UBA*0201,*0801,*0901 specific |
UBAlead2.F | AGCCCTACATTCTTCATCTGC | Exon 1 | Sasa-UBA*0301,*1001 specific |
UBA02.285R | GTTTGATTGAAGCGGGATTTC | Exon 2 | Sasa-UBA*0201 specific |
UBA03.281R | TCTGATTGAAGCGCTGCTTGG | Exon 2 | Sasa-UBA*0301 specific |
UBA08.280R | CTGATTGAAGCGCTGCTTGAC | Exon 2 | Sasa-UBA*0801 specfic |
UBA01.286R | GTTTGATTGAAACGTGTCTG | Exon 2 | Sasa-UBA*0901 specific |
UBA10.272R | TTGAAAGATTGTGAGGTGCT | Exon 2 | Sasa-UBA*1001 specific |
ssal.phc-004.039-2 | CTCTCCAGTGACCTGCCACGCTACAGGTTTCTATCCCAGTGGAGTCATGGTGTTCTGGCAGAAAGATGGA | MHC Non-classical | |
ssal.rgb-532.282-r | GCCAATGTGCTCATGGCTATAGTCATCATTGTGTCTGTAGTCTTGATACTCACTGTCCTATTCAAGTATT | MHC Non-classical | |
ssal.rgb-505.101 | GCTACCATCGGCTCAAAGAGGAACTCTTTGAGGACTGATTAGGAATCACACAGCTGTCAGAGAGAGAGAC | TAP2 | |
ssal.rgb-516.329 | TCAACCTGTACCACATGCAGGAGGACGGCTGGATAAAGGTGTGTAAGGAGGACGTTTCAGAGCTGATC | PSMB8 | |
ssal.rgb-523.298 | GTTGATGGACAGGAAGGGGAGCTACTACAAACTAAGAGAGAGACTGTTCACGGAAGACGACACGTCACAT | TAP2 | |
ssal.rgb-550.202 | TTCATTGGCTATGAGTCGAGATGGTTCCAGCGGAGGCGTGGCCTACCTTGTCACTATTGATGAAAAGGGT | PSMB9 | |
868O01_F1 | GGCTTGCGGAGTAGAACACTTGAAAAAGAA | GAP-primers | |
868O01_F2 | GCAACCAATACACTGCAGTATTTCTACACGGCCACTTCTGGTATAGATAAC | GAP-primers | |
868O01_F3 | TCAATCAAACTGGAGGTGAGTAGAGACAGA | GAP-primers | |
714P22_R1 | CAGCGGAAACGCAACAAACACAAGAATAACTTACTAACAAATTAGAATCA | GAP-primers | |
714P22_R2 | AGCATACAACCATGCCGACAACCATC | GAP-primers | |
92I04_R1 | AACATTTCAAGGTGGTGAAACTATTCACAT | GAP-primers | |
92I04_R2 | AATTAGTTGTTGTTCACTATGTTAATTAGT | GAP-primers |