Table 3 PCR verification of selected introns that were alternatively spliced
From: cDNA sequences reveal considerable gene prediction inaccuracy in the Plasmodium falciparum genome
Gene ID | Ch | Forward primer | Reversed primer | Gen | Spl | Comments |
|---|---|---|---|---|---|---|
PFB0260w | 2 | TCAAACACACGTTACACCT | ATGACAATACCTTCTAAGG | 242 | 102 | Confirmed |
PFB0305c | 2 | ACCTTTTGTTAATTATGGA | CCACCTTCTCCTTTTTCG | 255 | 135 | Confirmed |
PFB0177c | 2 | ACTAATGGTAGAATAGGTG | TTTCTCCATTTTGTATATCG | 373 | 161 | Confirmed |
PFB0535w | 2 | CAAAGATAAAATGGTAATGTT | ATTCCTATTATAGTGTGTGT | 507 | 297/243 | No 243-bp band |
PFC0371w | 3 | CCTACCTTCTATTTACAAAT | ACTTGTTGCTCTGATATAAT | 301 | 204 | No 204-bp band |
PFD0810w | 4 | GCTGTGAAAAAAGAAAACAA | TTGTTTTCTTTTTTCACAGC | 320 | 174 | No products |
PFD0895c | 4 | TTGATAACAATCCTTTAAGC | AATTCGTAATAATCATCTCC | 374 | 205 | Confirmed |
PFE1540w | 5 | GATCCTGAAATTGTTTGTG | ATGGCCAAAATGTTTCACA | 393 | 328/283/210 | Confirmed |
MAL8P1.81 | 8 | GCTGACATATTTATCTTATG | CATATAAGTATTCATGCATG | 303 | 147 | Confirmed |
PF10_0096 | 10 | ATATTATCGATATTGTCTATATTC | CTTGCTTTGTTTGGCTTCCA | 441 | 182 | Confirmed/antisense |
PF10_0170 | 10 | TATATTTGTCCTCAGTGC | CTTCCATATCAGATGCCA | 300 | 135 | 90-bp band, not 135 |
PF10_0017 | 10 | GGATAAATAGTTTTTTGCTT | CTCAGACAATGTACGCATA | 410 | 263 | Confirmed |
PF10_0117 | 10 | ATTGGAATTTAACTAGCAAC | TTCATAAGAGTGTTGTTCG | 330 | 134 | Confirmed |
PF10_0213 | 10 | GGTGCGAATAATAAAGTAG | CTACTTTGTTATTATCTCC | 349 | 229 | Add'l 150-bp band |
PF10_0247 | 10 | AATTACAAACAATTTGAGGG | TTCATTTTTCAAAAATGCGG | 383 | 152 | No 383-bp band |
PF10_0258 | 10 | AAAGACGAGGAACTTAATAC | CTCTGATTCTTTTATGAAAG | 270 | 150 | Confirmed |
PF10_0415 | 10 | CACCAATTTATAAAAGAAGAA | GGCAATAAAAAAGCCTGTTA | 370 | 183 | Confirmed |
PF11_0292 | 11 | AAGATGACCAACAAGAAGAA | TTATAGTACTCAATAACCTG | 340 | 153 | Confirmed |
PF11_0377 | 11 | CCGAAAAGGATAAGAAGAAG | TGATTATATGCTGCATATAC | 1425 | 168 | No 1425-bp band |
PF11_0167 | 11 | TAAGAAATTATGTTCCCAAT | TTTTTCTCCTACACAAGTGC | 354 | 152 | Confirmed |
PF11_0405 | 11 | TGAACTTAATACACATACGT | ACAGTATCTGAAGGATCTGT | 201 | 130 | No 130-bp band |
PFL0020w | 12 | TTCGATATATCATTCCATTC | AAACAGCTACTAGTTGTCC | 261 | 78 | No 78-bp band |
PFL0290w | 12 | CTTTATATTATCCAACAACAC | TTGTAATTACTTATAGGAGC | 454 | 167 | No 167-bp band |
PFL0580w | 12 | GATGCAATATTAGGTAGACT | ACTAAAGATTAGGTTAACAC | 294 | 193 | Confirmed |
PFL0890c | 12 | GAAATGCTCAACAAATTTGA | ACAGATATTATGGGAATTTC | 255 | 130 | Confirmed |
MAL13P1.130 | 13 | GTATCCAGAAATATTTTTTAC | GTATCAAAAATCCAACACGTA | 800 | 303 | No 303-bp band |
MAL13P1.183 | 13 | CTCCTAGAAATCCTAGATAT | GACTATGCAGTTTTTTTTATC | 315 | 311 | Add'l ~90-bp band |
MAL13P1.51 | 13 | CATTTATTGAATGCTCAGC | GTAGTAATATTCTCTCCTG | 180 | 46 | Confirmed |
MAL13P1.80 | 13 | CCAAAAAAGGACCTAATAAA | TATATATATGCACACGACAT | 376 | 219/150 | No 150 bp band |
PF13_0082 | 13 | CGAAGTGACAAAAAAAAGGA | CAGAATTTTTCCTATTATCG | 294 | 118 | Confirmed |
PF13_0224 | 13 | CTGATTTGTTTTTTCAACAAT | GAGTTATCTATTTTTTTAACC | 351 | 152 | Confirmed |
MAL13P1.195 | 13 | GAAAATGTCTGTCTTGTCAA | GCGTTCATATCGTCAAAAGA | 297 | 179 | Confirmed |
MAL13P1.253 | 13 | TTTTTACGAACAAAACGGTT | CTTTTGTTTGATCTAATACC | 215 | 117 | Confirmed |
PF13_0220 | 13 | AGTCATATCAAAAAATAGCT | GTACTTGTCTGATCTTTCTT | 284 | 123/167 | Confirmed |
PF13_0301 | 13 | AAAAATGAATGGAGTCCAGC | GCTGTTTTTAAATAAAGGGA | 243 | 146 | Confirmed |
PF14_0434 | 14 | GGATAGAAGAAACTATAACC | ATGCTATCATACTTACTGG | 206 | 104 | Confirmed |
PF14_0779 | 14 | CCTGATATGCGTGAAATT | TTTTTTCAATATTGTCGTACC | 525 | 90 | No 525-bp band |
PF14_0338 | 14 | AAAACAAGAATTTATCACGG | GATTCATTCCTGAATGGTCT | 727 | 116 | Confirmed |
PF14_0488 | 14 | AAAAAAAGGTCTACAAAAGC | TTGTTAAAATATTCCAAGGC | 230 | 92 | Confirmed |
PF14_0576 | 14 | GCACAATTTGAAAGAAAATT | ACTCGTGATGTAAATTTTCA | 629 | 230 | No 629-bp band |
PF14_0787 | 14 | CCTTTATTCATATGTGGAAT | GCAAGAGAAAATGGTTTAATAC | 585 | 120 | Add'l genomic bands |
PF14_0790 | 14 | GAATAGGAAAATATGCCAAG | GAATTATTACTATTCATCAC | 239 | 111 | Confirmed |