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Figure 1 | BMC Genomics

Figure 1

From: Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library

Figure 1

Testicular expression of the genes. (A) Complementary DNA from whole testes of wild-type (WT) mice and germ cell-lacking testes of W/Wv mutant mice were amplified by PCR. All the genes were expressed in wild-type testis but not testis deficient in germ cells, suggesting germ cell-specific expression. The faint bands observed in the germ cell-lacking testes for some of the genes are likely to be non-specific signals, since their sizes are different from those shown in WT testis. (B) Genes were analyzed by RT-PCR in eight adult male mice tissue types. All genes except Mm.425335 were exclusively expressed in the testis. The glyceraldehyde-3-phosphate dehydrogenase (Gapdh) band intensity was used to estimate equivalent amounts of cDNA templates among tissues. T, Testis; B, Brain; H, Heart; Li, Liver; Lu, Lung; K, Kidney; S, Spleen; M, Skeletal muscle.

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