Figure 4

Genomic, transcript, and protein characteristics of the genes in silico. Gene structure and exon organization were determined using genome database searches. In exon organization, the boxes represent exons. The bars indicate regions amplified in the PCR analysis and used as probes in the Northern blot analysis. Coding regions were determined by selecting the longest open reading frames deduced from cDNA sequences. The predicted coding regions are shaded. The position of the poly A signal is marked by filled arrowheads, and the presence of poly A is indicated by 'A'. Chromosomal location was determined by searching the mouse and human genome databases. The predicted amino acid sequences of genes were analyzed using various bioinformatics tools (see Experimental Procedure). For annotation of genes with ontology terms, amino acid sequences were submitted to and subsequently obtained from exclusive web servers (Goblet), which use a variety of different protein databases and provide gene ontology codes. Each gene ontology code falls into one of the larger categories of molecular function (M), cellular component (C), or biological process (B).