Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library

Choi, Eunyoung; Lee, Jiae; Oh, Jungsu; Park, Inju; Han, Cecil; Yi, Chongil; Kim, Do Han; Cho, Byung-Nam; Eddy, Edward M; Cho, Chunghee

doi:10.1186/1471-2164-8-256

BMC Genomics

Table 2 Analysis of unknown, testis-specific gene candidates

From: Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library

No	ID	Coding seq.	PCR		Northern	No	ID	Coding seq.	PCR		Northern
			E	T					E	T
1	Mm.46140	+	+	-		68	Mm.84572	+	+	+	+
2	Mm.153895	+	-			69	Mm.373242	+	+	+	+
3	Mm.85000	+	+	-		70	Mm.84567	+	+	-
4	Mm.67591	-				71	Mm.81022	+	+	+	-
5	Mm.425335	+	+	+	+	72	Mm.73639	-
6	Mm.45833	+	+	+	+	73	Mm.290718	+	+	+	+
7	Mm.26987	+	+	-		74	Mm.66965	+	+	-
8	Mm.232593	+	+	+	-	75	Mm.55870	+	+	+	+
9	Mm.157041	-				76	Mm.50068	+	+	+	-
10	Mm.152558	+	-			77	Mm.46472	-
11	Mm.152543	+	-			78	Mm.46159	+	+	+	+
12	Mm.138549	+	+	-		79	Mm.437189	+	+	+	+
13	Mm.107775	+	+	+	+	80	Mm.116803	+	+	+	+
14	Mm.86807	+	+	+	-	81	Mm.46144	+	-
15	Mm.86671	+	+	+	+	82	Mm.45824	+	+	+	+
16	Mm.63791	-				83	Mm.45377	+	-
17	Mm.5349	+	-			84	Mm.33810	-
18	Mm.157509	+	-			85	Mm.329058	+	+	+	+
19	Mm.116959	+	+	-		86	Mm.257647	-
20	Mm.87381	+	+	-		87	Mm.257295	-
21	Mm.87368	+	-			88	Mm.250945	+	+	+	-
22	Mm.85045	+	+	+	+	89	Mm.249832	+	+	-
23	Mm.333010	+	+	+	+	90	Mm.23578	-
24	Mm.263708	+	+	+	+	91	Mm.234186	-
25	Mm.72810	+	+	+	-	92	Mm.23377	+	+	+	+
26	Mm.67234	+	+	+	+	93	Mm.23356	+	+	+	-
27	Mm.660	+	+	-		94	Mm.233179	-
28	Mm.63782	+	+	-		95	Mm.230316	-
29	Mm.61136	-				96	Mm.228181	-
30	Mm.60992	+	+	-		97	Mm.216973	-
31	Mm.56177	+	+	+	-	98	Mm.210318	-
32	Mm.52285	+	-			99	Mm.210312	+	+	+	-
33	Mm.46124	+	+	-		100	Mm.195791	+	-
34	Mm.45462	+	+	-		101	Mm.271248	-
35	Mm.33629	+	+	+	-	102	Mm.165697	+	+	+	+
36	Mm.30958	+	+	+	-	103	Mm.191144	-
37	Mm.257958	+	+	-	-	104	Mm.188575	+	+	+	-
38	Mm.250904	-				105	Mm.176944	-
39	Mm.250670	-				106	Mm.175502	-
40	Mm.238261	+	-			107	Mm.172330	-
41	Mm.23534	+	+	+	+	108	Mm.172329	-
42	Mm.210311	+	-			109	Mm.172326	-
43	Mm.20109	-				110	Mm.172324	-
44	Mm.192428	+	+	-		111	Mm.171540	-
45	Mm.18549	+	-			112	Mm.159795	+	+	+	+
46	Mm.171269	+	-			113	Mm.159590	+	+	-
47	Mm.170672	-				114	Mm.158502	+	-	-
48	Mm.159587	-				115	Mm.158267	+	+	+	-
49	Mm.157881	-				116	Mm.158164	+	-
50	Mm.157632	+	-			117	Mm.157767	+	+	+	+
51	Mm.153685	+	-			118	Mm.157538	-
52	Mm.152610	-				119	Mm.157531	-
53	Mm.152605	+	+	-		120	Mm.157510	-
54	Mm.152599	+	+	-		121	Mm.157006	-
55	Mm.152597	+	-			122	Mm.152611	-
56	Mm.152594	-				123	Mm.152606	-
57	Mm.159281	+	+	+	+	124	Mm.152604	-
58	Mm.152587	+	-			125	Mm.152601	-
59	Mm.152586	-				126	Mm.152600	+	-
60	Mm.152544	-				127	Mm.152598	+	-
61	Mm.252733	+	+	+	+	128	Mm.152541	-
62	Mm.148821	+	-			129	Mm.139088	-
63	Mm.128120	-				130	Mm.132427	-
64	Mm.11014	-				131	Mm.109314	+	-
65	Mm.109877	-				132	Mm.108732	-
66	Mm.101915	+	-			133	Mm.103240	-
67	Mm.99172	-				134	Mm.101717	-

Gene candidates were subjected to in silico and in vitro analyses. Genes with potential coding sequences were analyzed by RT-PCR in which those expressed (E) as correctly-sized PCR products were further analyzed to determine whether they are testis-specific (T). Lastly, to investigate transcript size and expression level, genes with testis-specific expression were subjected to Northern blot analysis. Genes with positive and negative results are indicated as + and -, repectively.

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ISSN: 1471-2164

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