Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library

Choi, Eunyoung; Lee, Jiae; Oh, Jungsu; Park, Inju; Han, Cecil; Yi, Chongil; Kim, Do Han; Cho, Byung-Nam; Eddy, Edward M; Cho, Chunghee

doi:10.1186/1471-2164-8-256

BMC Genomics

Table 3 List of genes and gene specific primers designed for RT-PCR analysis

From: Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library

UniGene ID	GenBank ID	PCR primers		Size (bp)
		Forward (5'-3')	Reverse (5'-3')
Mm.329058^a	AB097085	AAGGCTTCTCTGTCGGATCG	TGCACTCCAGGTTCAAACAG	797
Mm.290718^a	DQ864732	TTGCAAGACTAGGGATGGTG	TGCCAGAGAGTGTGAGAGGG	588
Mm.157767	AK077190	ACTGTACCTGCTGTGACTGC	TTTGTGTTCAGGGTCTCCAG	979
Mm.46159^a	AK005850	CTGCAAGTTCCTGTGTTCTG	CAGGGATGACAAGGACAACG	767
Mm.55870	AK029548	TTCTCAGGCTTCCAGGTATG	GGGAGCAAGCCAGTAAGCAG	1025
Mm.45824^a	AK005610	GTCCCTCTGTAAGTCTGAAG	TGGACACTCTTTGGACACAG	538
Mm.333010	AK030264	GATTGCGACAGTTGACAGTG	TGATCCATTGTACAGACGGC	862
Mm.252733	AK006257	GCCGTGATCTCCAAAATGTC	ACAGCTTCAGTGCGTTCTTC	810
Mm.165697	AK006079	GGCTTTAGTGAGGACCTTGG	ACAGTCCCTAGCTCAGATTG	968
Mm.159281	XM_127913	TAACGGGAAGAAGGTGGAAG	AGGAGGGTCTGTAAGGTAGC	475
Mm.45833^a	NM_133711	AGATTGTTTACCACTGCCGG	CTACTTCCTCCATGTCCTTC	914
Mm.23534^a	AK016718	TTCCTCACGGCACAACTCAG	GTGTCCTCCGAATCCCTCAG	997
Mm.85045	AK019748	CCTGTATCTTCACTGCCTCG	AGTTCTCGTTTCAGCCAGGG	772
Mm.23377^a	AF522069	CCCTAGAGCTCTGTCCTTGG	CAGACATCAGCTCGGCCAAC	997
Mm.263708	AK006048	TCTCGGGCATCCTTCTGGGC	GGCTGAGAAGGTGTGGGCG	681
Mm.84572	AK006488	CCTTTTGGGGTGCACTTGGC	AGAGAGACAACCAAGTGAGC	1014
Mm.86671	AK133200	TTAAGTCCTGCTGCTGTTGG	ATGCCTCAAGGGAACTCCAG	440
Mm.425335	AK005943	TTCTTTCTCAGGTGCCGATG	CTTTCTGTGACATGTGACGC	254
Mm.67234	AK018916	AAGATGTCCTGTGGCCCTGG	GCCAGACAGAACAGGTAGTG	639
Mm.373242	AK077102	CAGCATAGGCGCAGAAAGAG	AGTCCCAGAGAAGAGGCCAG	683
Mm.437189^a	AF463501	CTATGACTCGGGTGGCCAAG	TGGCTTTGACAGGACCACAG	545
Mm.116803^a	AK014971	CTCTCTCACCTCCGTTCCAG	GAGTGAGAAAGGCAAGGAAG	1009
Mm.107775	AK015444	CATCTGCTCTGGTCCTGCTG	GACAACCCCATTCTATCCCG	876
Mm.159795^a	AK014942	TCGGGCTCACTGTTTGCTAC	TGAAGGAGAGACTAGTGCTG	964

^a The following genes were named previously: Mm.329058, Tudor domain containing 6 (Tdrd6) [47]; Mm290718, Zinc finger protein 541 (Zfp541); Mm.46159, Ring finger protein (Rnf151); Mm.45824, Spermatogenesis associated 19 (Spata19); Mm.45833, Spermatogenesis associated 4 (Spata4) [48]; Mm.23534, Tektin 3 (Tekt3) [23]; Mm.23377, Testis expressed gene 22 (Tep22) [22]; Mm.437189, Cysteine-rich perinuclear theca protein 4 (Cypt4) [20]; Mm116803, HRAS-like suppressor family, member 5 (Hrasls5); and Mm.159795, Cation channel, sperm associated 3 (CatSper3) [21].

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ISSN: 1471-2164

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