Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library

BMC Genomics

Table 4 Putative functions of the eight gene products in reproduction

Function	UniGene	In silico information	Localization in GC-2 cells	Presence in TSC	Presence in MS
Transcriptional regulation	Mm.290718	Transcription	Nu	n.d.	n.d.
	Mm.86671	DNA binding	Nu	n.d.	n.d.
	Mm.373242	Transcription	Nu	n.d.	n.d.
Nuclear integrity	Mm.437189	Perinuclear protein	Nu	n.d.	n.d.
Sperm structure or motility	Mm.23534	Tektin3	Cy	+	+ (T)
	Mm.23377	Tep22	n.d.	+	+
	Mm.159795	CatSper3	ER	n.d.	n.d.
Fertilization	Mm.333010	Trypsin-like serine protease	GA	+	+ (A)

Eight genes with in vitro protein data (Figs. 5, 6 and 7) which support in silico information (Table 1 and Figure 4) are functionally categorized. Abbreviations are as follows: TSC, testicular spermatogenic cells; MS, mature sperm; Nu, nucleus; Cy, cytoplasm; ER, endoplasmic reticulum GA, Golgi apparatus; n.d., not determined; (T), tail; (A), acrosome.

ISSN: 1471-2164