Immunodetection of selected candidate genes. (A) Immunoblots for ANXA2, AQP1, NRGN, and CANX was performed for three control and six diseased subjects. The size of quantified bands is indicated in kDa. The glycosylated form of AQP1 (*), undetectable in the healthy samples analyzed, was not included in the analysis. CANX served as a reference gene for sample normalization. (B) Bar graph comparing fold-changes of mean expression levels diseased vs. control as detected by immunoblot and microarray. Normalized intensity values are shown diseased vs. control and were analyzed by a two-tailed Student's t-test. P values for immunoblot and microarray (most significant probe) data were 0.025 and 0.011 (ANXA2), 0.055 and 0.036 (AQP1), and 0.047 and 0.023 (NRGN), respectively). (C) Immunofluorescence detection of AQP1 expression in non-neuronal cells in the diseased (D) and the healthy (H) motor cortex. NF – neurofilaments (red); AQP1 – aquaporin 1 (green); DNA – Hoechst33342 counterstain (blue). The scale bar indicates 100 μm.