Figure 1

Growth of S. coelicolor M600 and M600 ΔbldA in supplemented minimal medium. A schematic representation of the measured growth curves is shown with M600 denoted by a solid line, and M600 ΔbldA by a broken line. Points 1–8 indicate times when cultures were harvested for RNA and protein extraction, and the shaded arrow represents the onset of pigmented antibiotic production in M600 (the mutant did not produce pigments). All eight sampling points were analysed transcriptomically, while only samples 1, 2, 4, 6 and 8 were subjected to proteomic analysis.