Expression of a cluster of function-unknown genes is dependent on bldA. (A) Transcriptome and (B) proteome data showing the bldA-dependence of expression of the genes of unknown function SCO4251-4253, SCO4246, SCO4256 and SCO4262. (C) Genetic organisation of the SCO4251-4253 locus. (D) Transcription of the SCO4253 gene during growth of strains M600 and M600 Δ SCO4263 in SMM. RNA was isolated during the mid-exponential (E) and early stationary (S) phases of growth and subjected to S1 nuclease protection analysis using uniquely end-labelled PCR-generated probes. Transcription of the SCO0762 gene was analysed as an internal control for RNA quality and loading. (E) Sequence upstream of the SCO4253 gene indicating the location of the single transcription start site (bent arrow), upstream of the ATG translation start codon (block arrow).