|
A) Spots differ in isoelectric point (pI): spot 1 has more acidic pI than spot 2
|
|
SCO1246
|
BioD (dethiobiotin synthetase)
|
>2-fold up in ΔbldA
|
>2-fold down in ΔbldA
|
|
SCO1916
|
Putative transferase
|
Absent in ΔbldA
|
No change
|
|
SCO2390
|
FabF (3-oxoacyl- [ACP] synthase II
|
No change
|
>2-fold down in ΔbldA
|
|
SCO2618
|
ClpP2 (Clp-protease subunit 2)
|
>2-fold up in ΔbldA
|
>2-fold down in ΔbldA
|
|
SCO3073
|
HutU (urocanate hydratase)
|
No change
|
Absent in ΔbldA
|
|
SCO3137
|
GalE1 (UDP-glucose 4-epimerase)
|
Absent in ΔbldA
|
No change
|
|
SCO3409
|
Ppa (inorganic pyrophosphatase)
|
>2-fold up in ΔbldA
|
>2-fold down in ΔbldA
|
|
SCO4164
|
CysA (thiosulphate supfurtransferase)
|
>2-fold up in ΔbldA
|
>2-fold down in ΔbldA
|
|
SCO7400
|
ABC transport protein
|
>2-fold down in ΔbldA
|
No change
|
|
B) Spots differ in Mwt (and pI): spot 1 has higher Mwt than spot 2
|
|
SCO2271
|
Putative membrane protein
|
>2-fold down in ΔbldA
|
No change
|
|
SCO5465
|
Conserved hypothetical protein
|
Absent in ΔbldA
|
>2-fold up in ΔbldA
|
