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Table 2 Changes observed in the post-translational modification of proteins as a result of bldA mutation

From: New pleiotropic effects of eliminating a rare tRNA from Streptomyces coelicolor, revealed by combined proteomic and transcriptomic analysis of liquid cultures

Gene Function1 Alteration in protein abundance2 observed for:
   Spot 1 Spot 2
A) Spots differ in isoelectric point (pI): spot 1 has more acidic pI than spot 2
SCO1246 BioD (dethiobiotin synthetase) >2-fold up in ΔbldA >2-fold down in ΔbldA
SCO1916 Putative transferase Absent in ΔbldA No change
SCO2390 FabF (3-oxoacyl- [ACP] synthase II No change >2-fold down in ΔbldA
SCO2618 ClpP2 (Clp-protease subunit 2) >2-fold up in ΔbldA >2-fold down in ΔbldA
SCO3073 HutU (urocanate hydratase) No change Absent in ΔbldA
SCO3137 GalE1 (UDP-glucose 4-epimerase) Absent in ΔbldA No change
SCO3409 Ppa (inorganic pyrophosphatase) >2-fold up in ΔbldA >2-fold down in ΔbldA
SCO4164 CysA (thiosulphate supfurtransferase) >2-fold up in ΔbldA >2-fold down in ΔbldA
SCO7400 ABC transport protein >2-fold down in ΔbldA No change
B) Spots differ in Mwt (and pI): spot 1 has higher Mwt than spot 2
SCO2271 Putative membrane protein >2-fold down in ΔbldA No change
SCO5465 Conserved hypothetical protein Absent in ΔbldA >2-fold up in ΔbldA
  1. 1from ScoDB [55]
  2. 2protein abundance profiles for all 22 protein spots are detailed in Additional File 7.