Graphical representation of predicted sequence alignments between Fusarium graminearum reference sequence and Fusarium virguliforme BAC-end sequences. Inset key panel shows how color of predicted match was determined. A match from both ends of the BAC within 200 kb was required for display. Uni-directional matches are indicated in blue. Reversed order matches are indicated in green if they cross a scaffold marker (indicating a discontinuous sequence) and red if they are entirely within a single, continuous scaffold. Fusarium graminearum exonic fragments are indicated in red (5'-3') and magenta (3'–5'). Inset sequence alignment panel illustrates the strength of the match between both ends of clone “2M11” and scaffold 1.52 (1e-35 top and 3e-150, bottom) The presence of Hind III restriction fragments was verified in the F. graminearum sequence, as would be predicted by the position of 2M11.