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Table 2 Retrotransposition frequencies of wild-type and mutated tagged Zorro3 elements.

From: A yeast model for target-primed (non-LTR) retrotransposition

Transforming plasmid

Mutation

Retrotransposition frequency (URA3+ colonies/106 cells)A, B

Relative retrotransposition frequency (% of wild-type)

No. of assaysC

Individual retrotransposition frequencies

pZ3BN1

Wild-type

4.47

100

10 (8)

0.664, 0.882, 1.130, 1.631, 1.641, 1.783, 2.344, 2.989, 15.339, 16.368

pZ3BN2

ORF2 stop

0.20

4.4

8 (6)

0.037, 0.043, 0.051, 0.129, 0.148, 0.170, 0.211, 0.795

pZ3BN3

ORF2 frameshift

0.16

3.6

5 (5)

0.000, 0.033, 0.053, 0.250, 0.469

pZ3BN4

ORF2 ATG → ACG

0.20

4.5

4 (4)

0.021, 0.107, 0.333, 0.353

pZ3BN5

InterORF poly-A deletion

2.20

49.2

9 (7)

0.110, 0.262, 0.263, 0.290, 0.329, 0.345, 0.608, 1.875, 15.739

pZ3BN6

EN missense

0.09

1.9

5 (5)

0.000, 0.015, 0.065, 0.093, 0.257

pPZ3TAΔPA

Internal deletion

0.00

0.0

8 (6)

0.000, 0.000, 0.000, 0.000, 0.000, 0.000, 0.000, 0.000,

  1. A The numbers of URA3+ colonies were counted after 21 days incubation at 22°C.
  2. B No URA3+ colonies were ever observed with the parental strain, CAI4, under identical conditions.
  3. C The number of independent transformants analysed is shown in parentheses.