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Table 2 Retrotransposition frequencies of wild-type and mutated tagged Zorro3 elements.

From: A yeast model for target-primed (non-LTR) retrotransposition

Transforming plasmid Mutation Retrotransposition frequency (URA3+ colonies/106 cells)A, B Relative retrotransposition frequency (% of wild-type) No. of assaysC Individual retrotransposition frequencies
pZ3BN1 Wild-type 4.47 100 10 (8) 0.664, 0.882, 1.130, 1.631, 1.641, 1.783, 2.344, 2.989, 15.339, 16.368
pZ3BN2 ORF2 stop 0.20 4.4 8 (6) 0.037, 0.043, 0.051, 0.129, 0.148, 0.170, 0.211, 0.795
pZ3BN3 ORF2 frameshift 0.16 3.6 5 (5) 0.000, 0.033, 0.053, 0.250, 0.469
pZ3BN4 ORF2 ATG → ACG 0.20 4.5 4 (4) 0.021, 0.107, 0.333, 0.353
pZ3BN5 InterORF poly-A deletion 2.20 49.2 9 (7) 0.110, 0.262, 0.263, 0.290, 0.329, 0.345, 0.608, 1.875, 15.739
pZ3BN6 EN missense 0.09 1.9 5 (5) 0.000, 0.015, 0.065, 0.093, 0.257
pPZ3TAΔPA Internal deletion 0.00 0.0 8 (6) 0.000, 0.000, 0.000, 0.000, 0.000, 0.000, 0.000, 0.000,
  1. A The numbers of URA3+ colonies were counted after 21 days incubation at 22°C.
  2. B No URA3+ colonies were ever observed with the parental strain, CAI4, under identical conditions.
  3. C The number of independent transformants analysed is shown in parentheses.