A yeast model for target-primed (non-LTR) retrotransposition

BMC Genomics

Table 3 Comparisons of relative retrotransposition frequencies and RNA levels for Zorro3 mutants

Transforming plasmid	Mutation	Relative retrotransposition frequency (% of wild-type)^A	Relative Zorro3 RNA levels (% of wild-type)^B
pZ3BN1	Wild-type	100	100
pZ3BN2	ORF2 stop	8	288
pZ3BN3	ORF2 frameshift	1	209
pZ3BN4	ORF2 ATG → ACG	18	130
pZ3BN5	InterORF poly-A deletion	15	98
pZ3BN6	EN missense	5	55
pPZ3TAΔPA	Internal deletion	0	34

^A The relative retrotransposition frequency was calculated in the same way as for Table 2, but data was taken from only the particular transformants in which RNA levels were also measured.
^B Zorro3 RNA levels were measured as described in the text. The Zorro3 RNA levels in each RNA sample were then normalized against the ACT1 RNA levels in the same RNA sample and averaged for each set of transformants containing the same plasmid construct.

ISSN: 1471-2164