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Table 2 Average expression ratios (DS samples/controls) calculated from microarray and RT-PCR data and Pearson correlation coefficient (r) between microarray and RT-PCR data. Hsa21 genes are in bold.

From: Altered expression of mitochondrial and extracellular matrix genes in the heart of human fetuses with chromosome 21 trisomy

Functional Category Probe ID Gene Name GenBank Micro-array Fold change Micro-array p-value RT-PCR ratio r Primers for RT-PCR
Oxydative Phospho-rylation 201226_at NDUFB8 NM_005004 0.65 0.0160 0.76 0.78 LEFT-GCCAAGAAGTATAATATGCGTGTG
RIGHT-GTCAGGGAGCTTCGGGTAG
  201740_at NDUFS3 NM_004551 0.76 0.0294 0.60 0.88 LEFT-GATTATGGCTTCGAGGGACA
RIGHT-ACCCGCTTCACTTCATCATC
  201903_at UQCRC1 NM_003365 0.77 0.0142 0.89 0.84 LEFT-CCGAGCAGTCCTCTCAGC
RIGHT-TGTTCCCTTGAAAGCCAGAT
  201966_at NDUFS2 NM_004550 0.67 0.0010 0.58 0.89 LEFT-GAATGGGCACAGCAGTTTG
RIGHT-GGCCCAAAGTTCAGGGTAAT
  203606_at NDUFS6 NM_004553 0.75 0.0464 0.63 0.84 LEFT-AGAAGGTCACGCACACTGG
RIGHT-CACGGGCTGCTCTGCTAT
  203663_s_at COX5A NM_004255 0.79 0.0400 1.04 0.79 LEFT-AACTGGGCCTTGACAAAGTG
RIGHT-GGTAACTGTTCACACTCAAGTAGCA
  203858_s_at COX10 NM_001303 0.77 0.0101 0.66 0.92 LEFT-CTTTTGACTGGCCCTGTTTC
RIGHT-ACCAGCGGTCTGTTCTTTGT
  218160_at NDUFA8 NM_014222 0.73 0.0445 0.71 0.94 LEFT-GTCATGCCGGGGATAGTG
RIGHT-TTAAGCACAGCAGAACTAATTTTCA
  218190_s_at UCRC NM_013387 0.76 0.0017 0.76 0.89 LEFT-GACGCTATCTACGACCACATCA
RIGHT-GGTCCTTCTGGCCTGGAT
Extra-cellular Matrix 201069_at MMP2 NM_004530 1.59 0.0069 1.89 0.93 LEFT-TCCACCACCTACAACTTTGAGA
RIGHT-AACTTGCAGGGCTGTCCTT
  202310_s_at COL1A1 K01228 1.55 0.0333 5.86 0.90 LEFT-TGTTCAGCTTTGTGGACCTC
RIGHT-CTGTACGCAGGTGATTGGTG
  202403_s_at COL1A2 NM_000089 1.60 0.0192 3.59 0.77 LEFT-CACATGCCGTGACTTGAGAC
RIGHT-TAGCATCCATAGTGCATCCTTG
  202994_s_at FBLN1 Z95331 1.72 0.0271 2.38 0.89 LEFT-GCCACAAGTGCGAGAACAC
RIGHT-TAGACGTTGGCACACTCCTG
  209081_s_at COL18A1 AF018081 1.57 0.0079 1.91 0.95 LEFT-GTGGCCCTCTACGTGGACT
RIGHT-TCTGAGTCATCGCCTTCCTC
  213428_s_at COL6A1 AA292373 1.62 0.0493 2.20 0.78 LEFT-AGGAGACCCTGGTGAAGCTG
RIGHT-AGGTCCTGGGGCTCCTCT
  209156_s_at COL6A2 AY029208 2.39 0.0002 2.18 0.79 LEFT-GACCTGGTCGCTGAGAAGTT
RIGHT-GCCTTGTGGAAGTTCTGCTC
Other genes 205548_s_at BTG3 NM006806 1.68 0.0134 1.62 0.83 LEFT-GAGGCAGTTGAGAGGTTTGC
RIGHT-GAGTGAGCTCCTTTGGCAAG
  35776_at ITSN1 AF064243 1.72 0.0003 1.57 0.88 LEFT-GTGAGCGGCACTGATTTGT
RIGHT-GATCATGCTTCGCTCTTTCC
  205593_s_at PDE9A NM_002606 2.05 0.0067 2.05 0.89 LEFT-CAGAACGCACTCCGTACAAA
RIGHT-TGGGCTCTACCTGTCCACTT
  211065_x_at PFKL BC006422 1.42 0.0088 1.97 0.81 LEFT-GGTGGACCTGGAGAAGCTG
RIGHT-TCCAGGCGGAGTCAATGT
  200642_at SOD1 NM_000454 1.16 >0.05 0.65 0.83 LEFT-GCATCATCAATTTCGAGCAG
RIGHT-CAGCCTCTGTATTATCTCCAA
  203405_at DSCR2 NM_003720 1.15 >0.05 0.85 0.78 LEFT-AAAGACTCGGCGTGTTGTC
RIGHT-GAATTGCTGGGATTTTCCAT
  202671_s_at PDXK NM_003681 1.12 >0.05 1.00 0.98 LEFT-CATACAGAGCCACGTCATCC
RIGHT-GCATAGCCTGTGTGGTTTGA
  202325_s_at ATP5J NM_001685 1.00 >0.05 1.17 0.78 LEFT-TGTTTGGCTTCTGTCTCACC
RIGHT-GGCTGACCGAATGACAGAG
  202749_at WRB NM_004627 1.17 >0.05 1.25 0.85 LEFT-CTCAGCTTCGTGTTTGGATG
RIGHT-ACTGTGGAGAGCTCCTGCTT
  209033_s_at DYRK1A D86550 1.53 0.0001 1.71 0.84 LEFT-GATATCATATGGGTCAGGTCATTTT
RIGHT-CTGGACTGTAACATAACACAGTATGC
  208370_s_at DSCR1 NM_004414 1.25 >0.05 4.35 0.83 LEFT-TTTGCTCAGACCTTACACATAGGA
RIGHT-GGGAGGGGAGATCAGAAACT
  210555_s_at NFATC3 U85430 0.45 >0.05 0.54 0.97 LEFT-CTTTGCAATGGCAAGAGGA
RIGHT-GATGAGGCACAGGCAAAGAT
  217526_at NFATC2 AI478300 0.77 0.019 0.67 0.84 LEFT-GAGTTCACATCCCAGAGTCCA
RIGHT-GAGCACTCGATGGGGTTAGA