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Table 2 Average expression ratios (DS samples/controls) calculated from microarray and RT-PCR data and Pearson correlation coefficient (r) between microarray and RT-PCR data. Hsa21 genes are in bold.

From: Altered expression of mitochondrial and extracellular matrix genes in the heart of human fetuses with chromosome 21 trisomy

Functional Category

Probe ID

Gene Name

GenBank

Micro-array Fold change

Micro-array p-value

RT-PCR ratio

r

Primers for RT-PCR

Oxydative Phospho-rylation

201226_at

NDUFB8

NM_005004

0.65

0.0160

0.76

0.78

LEFT-GCCAAGAAGTATAATATGCGTGTG

RIGHT-GTCAGGGAGCTTCGGGTAG

 

201740_at

NDUFS3

NM_004551

0.76

0.0294

0.60

0.88

LEFT-GATTATGGCTTCGAGGGACA

RIGHT-ACCCGCTTCACTTCATCATC

 

201903_at

UQCRC1

NM_003365

0.77

0.0142

0.89

0.84

LEFT-CCGAGCAGTCCTCTCAGC

RIGHT-TGTTCCCTTGAAAGCCAGAT

 

201966_at

NDUFS2

NM_004550

0.67

0.0010

0.58

0.89

LEFT-GAATGGGCACAGCAGTTTG

RIGHT-GGCCCAAAGTTCAGGGTAAT

 

203606_at

NDUFS6

NM_004553

0.75

0.0464

0.63

0.84

LEFT-AGAAGGTCACGCACACTGG

RIGHT-CACGGGCTGCTCTGCTAT

 

203663_s_at

COX5A

NM_004255

0.79

0.0400

1.04

0.79

LEFT-AACTGGGCCTTGACAAAGTG

RIGHT-GGTAACTGTTCACACTCAAGTAGCA

 

203858_s_at

COX10

NM_001303

0.77

0.0101

0.66

0.92

LEFT-CTTTTGACTGGCCCTGTTTC

RIGHT-ACCAGCGGTCTGTTCTTTGT

 

218160_at

NDUFA8

NM_014222

0.73

0.0445

0.71

0.94

LEFT-GTCATGCCGGGGATAGTG

RIGHT-TTAAGCACAGCAGAACTAATTTTCA

 

218190_s_at

UCRC

NM_013387

0.76

0.0017

0.76

0.89

LEFT-GACGCTATCTACGACCACATCA

RIGHT-GGTCCTTCTGGCCTGGAT

Extra-cellular Matrix

201069_at

MMP2

NM_004530

1.59

0.0069

1.89

0.93

LEFT-TCCACCACCTACAACTTTGAGA

RIGHT-AACTTGCAGGGCTGTCCTT

 

202310_s_at

COL1A1

K01228

1.55

0.0333

5.86

0.90

LEFT-TGTTCAGCTTTGTGGACCTC

RIGHT-CTGTACGCAGGTGATTGGTG

 

202403_s_at

COL1A2

NM_000089

1.60

0.0192

3.59

0.77

LEFT-CACATGCCGTGACTTGAGAC

RIGHT-TAGCATCCATAGTGCATCCTTG

 

202994_s_at

FBLN1

Z95331

1.72

0.0271

2.38

0.89

LEFT-GCCACAAGTGCGAGAACAC

RIGHT-TAGACGTTGGCACACTCCTG

 

209081_s_at

COL18A1

AF018081

1.57

0.0079

1.91

0.95

LEFT-GTGGCCCTCTACGTGGACT

RIGHT-TCTGAGTCATCGCCTTCCTC

 

213428_s_at

COL6A1

AA292373

1.62

0.0493

2.20

0.78

LEFT-AGGAGACCCTGGTGAAGCTG

RIGHT-AGGTCCTGGGGCTCCTCT

 

209156_s_at

COL6A2

AY029208

2.39

0.0002

2.18

0.79

LEFT-GACCTGGTCGCTGAGAAGTT

RIGHT-GCCTTGTGGAAGTTCTGCTC

Other genes

205548_s_at

BTG3

NM006806

1.68

0.0134

1.62

0.83

LEFT-GAGGCAGTTGAGAGGTTTGC

RIGHT-GAGTGAGCTCCTTTGGCAAG

 

35776_at

ITSN1

AF064243

1.72

0.0003

1.57

0.88

LEFT-GTGAGCGGCACTGATTTGT

RIGHT-GATCATGCTTCGCTCTTTCC

 

205593_s_at

PDE9A

NM_002606

2.05

0.0067

2.05

0.89

LEFT-CAGAACGCACTCCGTACAAA

RIGHT-TGGGCTCTACCTGTCCACTT

 

211065_x_at

PFKL

BC006422

1.42

0.0088

1.97

0.81

LEFT-GGTGGACCTGGAGAAGCTG

RIGHT-TCCAGGCGGAGTCAATGT

 

200642_at

SOD1

NM_000454

1.16

>0.05

0.65

0.83

LEFT-GCATCATCAATTTCGAGCAG

RIGHT-CAGCCTCTGTATTATCTCCAA

 

203405_at

DSCR2

NM_003720

1.15

>0.05

0.85

0.78

LEFT-AAAGACTCGGCGTGTTGTC

RIGHT-GAATTGCTGGGATTTTCCAT

 

202671_s_at

PDXK

NM_003681

1.12

>0.05

1.00

0.98

LEFT-CATACAGAGCCACGTCATCC

RIGHT-GCATAGCCTGTGTGGTTTGA

 

202325_s_at

ATP5J

NM_001685

1.00

>0.05

1.17

0.78

LEFT-TGTTTGGCTTCTGTCTCACC

RIGHT-GGCTGACCGAATGACAGAG

 

202749_at

WRB

NM_004627

1.17

>0.05

1.25

0.85

LEFT-CTCAGCTTCGTGTTTGGATG

RIGHT-ACTGTGGAGAGCTCCTGCTT

 

209033_s_at

DYRK1A

D86550

1.53

0.0001

1.71

0.84

LEFT-GATATCATATGGGTCAGGTCATTTT

RIGHT-CTGGACTGTAACATAACACAGTATGC

 

208370_s_at

DSCR1

NM_004414

1.25

>0.05

4.35

0.83

LEFT-TTTGCTCAGACCTTACACATAGGA

RIGHT-GGGAGGGGAGATCAGAAACT

 

210555_s_at

NFATC3

U85430

0.45

>0.05

0.54

0.97

LEFT-CTTTGCAATGGCAAGAGGA

RIGHT-GATGAGGCACAGGCAAAGAT

 

217526_at

NFATC2

AI478300

0.77

0.019

0.67

0.84

LEFT-GAGTTCACATCCCAGAGTCCA

RIGHT-GAGCACTCGATGGGGTTAGA

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