Figure 1

Representative 2D gels of Macaca mulatta protein sample stained with SyproRuby™. The polypeptide molecular mass scale in kDa is depicted on the y-axis while the x-axis shows the pI range. The proteins were resolved in 4–7 linear pH gradient (Immobiline DryStrips; 240 × 3 × 0.5) and 8–15% gradient SDS-PAGE (2400 × 2000 × 1 mm). The results of the proteins that were identified (indicated by arrows) by in-gel trypsin digestion and MALDI-TOF-TOF followed by de novo sequencing are elaborated in Table 1.