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Table 1 The proteins identified by de novo amino acid sequencing using MALDI-TOF-TOF

From: De novo protein sequence analysis of Macaca mulatta

# Precursor ion (m/z) De novo peptide sequence Homology search peptide sequence [PEAKS()/SPIDER()] Score %() arbitrary() Sequence assigned to protein (Species)
1 1042.5265
1667.8766
LVTFYEDR
VPAFLSAAEVEEHLR
LVTFYEDR()
VPAFLSAAEVEEHLR()
97.81()
99()
Crystallin, mu (Homo Sapiens)
2 1631.7706 FQNIDFAEEVYTR FQNIDFAEEVYTR() 99() Guanine aminohydrolase(Homo Sapiens)
3 1303.7262 VLTPELYAELR
GTGGVDTAAVGGVFDVSNADR
VLTPELYAELR()
GTGGVDTAAVGGVFDVSNADR ()
99()
12.3()
Brain creatine kinase (Homo Sapiens)
4 1516.6975
1954.0564
[RT]YDESGPS[L]VHR
VAPEEHPVLLTEAPLNPK
[QE]YDESGPS[I]VHR()
VAPEEHPVLLTEAPLNPK()
7.6()
11.2()
Actin beta (Homo Sapiens)
5 1967.8951
1648.6646
[RS]A[L]QAAHDAVAQEGQCR
[N]GHLYELDGR
[NE]A[I]QAAHDAVAQEGQCR()
[D]GHLYELDGR()
10.0()
5.8()
Ubiquitin carboxyl-terminal esterase L1 (Homo Sapiens)
6 1211.6733 Q [L]TVNDL[VP]GR
EGGLGPLNIPLLADVTR
Q[I]TVNDL[PV]GR()
EGGLGPLNIPLLADVTR()
5.6()
95.97()
Peroxiredoxin 2 isoform b (Homo Sapiens)
7 1684.9053
2560.2588
AAVEEGIVLGGGCALLR
LVQDVANNTNEEAGDGTTTATVLAR
AAVEEGIVLGGGCALLR()
LVQDVANNTNEEAGDGTTTATVLAR()
98.28()
98.49()
Heat shock 60 kD protein 1 (Homo Sapiens)
8 1987.0314 AIAELGIYPAVDPLDSTSR AIAELGIYPAVDPLDSTSR() 11.2() ATP synthase beta chain, mitochondrial precursor (Homo Sapiens)
9 1917.9341 FQDGDLTLYQSNTFLR FQDGDLTLYQSNTFLR() 96.24() GST pi enzyme (Macaca mulatta)
10 1256.55 NCSETQYERK NCSETQYERK() 99.1() 14-3-3 gamma protein (Homo Sapiens)
11 1253.6162
1981.9982
FEELNADLFR
TVTNAVVTVPAYFNDSQR
FEELNADLFR()
TVTNAVVTVPAYFNDSQR()
97.95()
99.49()
Heat shock 70 kDa protein 8 isoform 1(Homo Sapiens)
12 1917.9427 SIQEIQELDKDDESLR SIQEIQELDKDDESLR() 99.17() Rho GDI alpha (Homo Sapiens)
13 1337.7222 PPYTVVYFPVR PPYTVVYFPVR() 98.1() Glutathione S-transferase