Figure 4

WASP SVG graphical user input interface. (a) WASP first page containing three input sections: 1) keyword input for local SNP db, 2) user-defined input and 3) AS primer parameter adjustment. (b) CYP2D6 gene output resulting from using CYP2D6 as a keyword search. The general information including gene locus, gene type, OMIM Id and related disease are reported here. Polymorphism related information on CYP2D6 gene is also shown e.g. number of SNPs in CYP2D6 gene according to dbSNP, HapMap and JSNP. (c) Gene structure view of CYP2D6 displayed as SVG image. The top ruler displays positions and a direction of the gene relative to the reference Contig and chromosome respectively. (d) Detailed information of each SNP including flanking DNA sequences, observed alleles, allele frequency, position on Contig/chromosome, amino acid change and its position relative to the transcription. (e) Selectable SNP list for AS primer design. Using the previously described information, users can select a set of SNP from this table for designing AS primers.