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Table 3 Specificity of the PRI-lock probe system in conventional real-time PCR and in the Biotrove OpenArray™ for non-target organisms with closely related ligation sites.

From: Quantitative multiplex detection of plant pathogens using a novel ligation probe-based system coupled with universal, high-throughput real-time PCR on OpenArrays™

  Pythium undulatum cnt Phytophthora spp.(1) Phytophthora sojae cnt P. infestans (3) Myrothecium verrucaria cnt M. roridum (5)
PRI-lock Probe CT(AB) CT(BT) CT(AB) CT(BT) CT(AB) CT(BT)
Phytophthora spp. nd nd 15.5 (0.11) 16.1 (0.10) nd nd
P. infestans nd nd nd nd nd nd
M. roridum nd nd nd nd nd nd
  1. The PRI-lock probes were ligated on 106 targets/μl ligation mixture. CT values were normalized using the ILC control PRI-lock probe. Data represent average CT values of three PCR replicates in the conventional real-time platform (n = 3) and of four PCR replicates in the Biotrove OpenArray™ (n = 4). Standard deviations are indicated between brackets. AB: samples run on the conventional real-time PCR platform. BT: Samples tested on the Biotrove OpenArray™ platform. Number of discriminating nucleotides in the ligation region are indicated between brackets. nd: not detectable. cnt: closest non target.