Table 4 Quantification and sensitivity of the developed assay on the Biotrove OpenArray™ platform.
PRI-lock probe | Calibration curve formula | R2 value | Detection limit (copy number/μl ligation mixture) | Linear quantification range (copy number/μl ligation mixture) |
|---|---|---|---|---|
Phytophthora spp. | y = -3.61x + 38.1 | 0.995 | 104 | 108 – 104 |
P. infestans | y = -3.31x + 35.7 | 0.989 | 103 | 108 – 103 |
R. solani AG 2-2 | y = -3.49x + 37.9 | 0.994 | 104 | 108 – 104 |
R. solani AG 4-1 | y = -3.49x + 35.8 | 0.994 | 103 | 109 – 103 |
R. solani AG 4-2 | y = -3.35x + 39.2 | 0.998 | 103 | 108 – 104 |
F. oxysporum | y = -3.47x + 39.1 | 0.990 | 104 | 108 – 104 |
M. roridum | y = -3.15x + 36.6 | 0.984 | 104 | 109 – 104 |
V. dahliae | y = -3.42x + 36.3 | 0.996 | 103 | 108 – 103 |
V. alboatrum/V. tricorpus | y = -3.40x + 38.8 | 0.991 | 103 | 108 – 104 |
M. hapla | y = -3.39x + 38.1 | 0.989 | 104 | 108 – 104 |
E. carotovora carotovora | y = -3.51x + 36.3 | 0.997 | 103 | 109 – 103 |
A. tumefaciens | y = -3.36x + 36.5 | 0.995 | 103 | 108 – 103 |
G. Proteo bacterial spp | y = -3.51x + 38.1 | 0.995 | 104 | 108 – 104 |