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Table 4 Quantification and sensitivity of the developed assay on the Biotrove OpenArray™ platform.

From: Quantitative multiplex detection of plant pathogens using a novel ligation probe-based system coupled with universal, high-throughput real-time PCR on OpenArrays™

PRI-lock probe Calibration curve formula R2 value Detection limit (copy number/μl ligation mixture) Linear quantification range (copy number/μl ligation mixture)
Phytophthora spp. y = -3.61x + 38.1 0.995 104 108 – 104
P. infestans y = -3.31x + 35.7 0.989 103 108 – 103
R. solani AG 2-2 y = -3.49x + 37.9 0.994 104 108 – 104
R. solani AG 4-1 y = -3.49x + 35.8 0.994 103 109 – 103
R. solani AG 4-2 y = -3.35x + 39.2 0.998 103 108 – 104
F. oxysporum y = -3.47x + 39.1 0.990 104 108 – 104
M. roridum y = -3.15x + 36.6 0.984 104 109 – 104
V. dahliae y = -3.42x + 36.3 0.996 103 108 – 103
V. alboatrum/V. tricorpus y = -3.40x + 38.8 0.991 103 108 – 104
M. hapla y = -3.39x + 38.1 0.989 104 108 – 104
E. carotovora carotovora y = -3.51x + 36.3 0.997 103 109 – 103
A. tumefaciens y = -3.36x + 36.5 0.995 103 108 – 103
G. Proteo bacterial spp y = -3.51x + 38.1 0.995 104 108 – 104
  1. (x) = Log10(copy number target input/μl ligation mixture). (y) = CT value.