value versus standard deviation for the reference RNA sample. Plotted here is the average CT value for each gene versus the standard deviation (SD) for each gene from a total of 11 assay runs. The "△" symbols represent genes where a single run had an empty assay well and was assigned a value of zero. The "□" symbol represents a gene where one out of the 11 runs resulted in a spurious "failed" well and was assigned a CT value of 40. These data show great consistency over the study.