Figure 2

Expression profiles of HwHog-ChIP positive genes in adapted and stressed H. werneckii cells and ChIP results in adapted cells. RT-PCR was performed with RNA isolated from adapted cells (Adaptation) or cells exposed to hypersaline stress (Hyperosmotic stress) as indicated. 26S rRNA (Hw26SRR) was used as an internal control for template normalization. The HwHog1-ChIP and sequential RNA polymerase II/HwHog1-ChIP (SeqChIP) results for cells adapted to 3 M NaCl and 4.5 M NaCl are presented in parallel. The negative control for nonspecific binding of antibodies in both ChIP experiments is represented in the "NoAB" line and the positive control for genomic DNA amplification from 100-fold diluted input samples is labeled as "INPUT". HwCOB1 and 26SRR genes were negative controls and HwGPD1A was the positive control for HwHog1 and RNA polymerase II cross-linking.