Skip to main content

Table 1 Differentially expressed genes in H. werneckii adaptation to 3 M NaCl or 4.5 M NaCl. BLASTX matches of EST clones derived from the Hw4.5-3 subtraction library and their functional categorizations based on MIPS are collected. Similarities with probability <10-5 were regarded as being significant, and others as not determined (ND). Genes selected for the expression and chromatin immunoprecipitation analyses are printed in bold. aGenBank accession number. bNumbers indicate the functional groups (see Table 3). Numbers in brackets correspond to literature-based arbitrarily-assigned functional categories for ESTs with orthologs in organisms other than S. cerevisiae. cFold induction (fI, positive value) or fold repression (fR, negative value) in 4.5 M NaCl- vs. 3 M NaCl-adapted H. werneckii cells. Numbers indicated are mean values of three independent RT-PCR experiments, and the representative gels are shown in figures 1 and 2.

From: Differential gene expression and Hog1 interaction with osmoresponsive genes in the extremely halotolerant black yeast Hortaea werneckii

GenBank.a Gene Characteristic or description Functional categoryb fI/f Rc
DQ822684 HwAGP1 Amino acid permease 20. 3.3
DQ822624 HwATP1 Mitochondrial ATPase alpha-subunit 2. 20. 34. 1.8
DQ822625 HwATP2 Mitochondrial ATPase beta-subunit 2. 20. 34. 40. 4.0
DQ822626 HwATP3 Mitochondrial ATPase gamma-subunit 2. 20. 34. 2.3
DQ822606 HwBMH1 14-3-3 protein 1. 10. 16. 30. 40. 43. 1.8
DQ822642 HwCDC3 Septin complex component 10. 16. 34. 40. 42. 43. -3.3
DQ822620 HwCIT1 Mitochondrial citrate synthase 1. 2. 42. 4.6
DQ822622 HwCOB1 Cytochrome b 2. 5.2
DQ822621 HwCOX1 Cytochrome c oxidase subunit I 2. 20. 2.5
DQ822687 HwCYT1 Cytochrome c1 2. 20. 3.6
DQ822643 HwDBP2 RNA helicase 1. 11. -4.2
DQ822641 HwECM33 Extracellular matrix 33 protein 10. 42. 43. 2.0
DQ822657 HwEFT2 Eukaryotic translation elongation factor 2 (eEF-2) 12. 1.9
DQ822685 HwELF1 Transcription elongation factor 10. 11. 40 7.6
DQ822613 HwERV25 p24 component of the COPII-coated vesicles 20. 9.4
DQ822619 HwFAS1 Mitochondrial acyl-carrier protein 1. 3.1
DQ822689 HwFRE7 Ferric-chelate reductase-7 transmembrane component 20. 34. 10.5
DQ822656 HwFUN12 Eukaryotic translation initiation factor 5B (eIF-5B) 12. 3.4
DQ822655 HwGAL10 UDP-glucose 4-epimerase 1. ND
DQ822610 HwGDH1 NADP(+)-specific glutamate dehydrogenase 1. 2. 16. 7.5
DQ822654 HwGND2 6-phosphogluconate dehydrogenase 1. 2. 16. 4.1
DQ822631 HwGUT2 FAD-dependent glycerol-3-phosphate dehydrogenase 1. 2. 42. 3.0
DQ822645 HwHHF1 Histone H4 10. 11. 16. ND
DQ822614 HwHSP82 Heat shock protein 90 1. 2. 10. 14. 32. 34. 43. 5.1
DQ822646 HwHTA1 Histone H2A 10. 11. 16. -2.6
DQ858164 HwIRE1 Serine-threonine protein kinase and endoribonuclease 1. 11. 14. 30. 32. 2.1
DQ822615 HwKAR2 Endoplasmic reticulum luminal chaperone 1. 10. 14. 16. 20. 32. 41. 42. 2.3
DQ822627 HwKGD2 Dihydrolipoamide succinyltransferase 1. 2. 1.8
DQ822629 HwLSC2 Mitochondrial succinyl-CoA ligase beta-chain 1. 2. 16. 2.7
DQ822630 HwMDH1 Mitochondrial malate dehydrogenase 1. 2. 42. 3.4
DQ858163 HwMET14 ATP adenosine-5'-phosphosulfate 3'-phosphotransferase 1. 3.4
DQ822607 HwMET17 Cystein synthase 1. 8.2
DQ822605 HwMET6 Cobalamin-independent methionine synthase 1. 3.2
DQ822628 HwMIR1 Mitochondrial phosphate transport protein 20. 34. 8.4
DQ822649 HwNHP6A High mobility group protein A 10. 11. 40. 43. 4.5
DQ822623 HwNUC1 Mitochondrial nuclease 1. 10. 3.2
DQ822635 HwOPI3 Unsaturated phospholipid methyltransferase 1. 9.7
DQ822612 HwPDI1 Protein disulphide isomerase precursor 14. 20. 2.1
DQ858158 HwPGK1 3-phosphoglycerate kinase 1. 2. 16. 6.4
DQ822634 HwPMA2 Plasma membrane proton-exporting ATPase 2. 20. 34. 7.9
DQ822651 HwPRY1 Pathogenesis-related protein precursor 34. 7.5
DQ822686 HwPUF1 Serine rich pumilio family RNA binding domain protein 1. 10. 11. 16. 4.6
DQ822688 HwRAD16 SWI/SNF family DEAD/DEAH box helicase 10. 32. 4.9
DQ822660 HwRPL10 60S ribosomal protein 10 10. 12. 14. ND
DQ822672 HwRPL16A 60S ribosomal protein 16A 12. 16. ND
DQ822664 HwRPL22A 60S ribosomal protein 22A 12. ND
DQ822670 HwRPL2B 60S ribosomal protein 2B 12. ND
DQ822671 HwRPL3 60S ribosomal protein 3 12. ND
DQ822669 HwRPL6A 60S ribosomal protein 6A 12. 16. 2.6
DQ822661 HwRPL7B 60S ribosomal protein 7B 12. ND
DQ822617 HwRPN2 26S proteasome regulatory particle subunit 11. 40. 30. 2.2
DQ822673 HwRPS10B 40S ribosomal protein 10B 12. ND
DQ822666 HwRPS12 40S ribosomal protein 12 12. ND
DQ822665 HwRPS15 40S ribosomal protein 15 12. 14. 20. ND
DQ822667 HwRPS16A 40S ribosomal protein 16A 12. 7.6
DQ822663 HwRPS17A 40S ribosomal protein 17A 12. ND
DQ822668 HwRPS26B 40S ribosomal protein 26B 12. ND
DQ822659 HwRPS8A 40S ribosomal protein 8A 12. ND
DQ822662 HwRPS8B 40S ribosomal protein 8B 12. ND
DQ822608 HwSAM2 S-adenosylmethionine synthetase 1. 2.8
DQ822633 HwSHY1 Mitochondrial inner membrane protein chaperone 2. 16. 32. 2.5
DQ858161 HwSKN1 Beta-1,6-glucan synthetase 1. 2. 42. 2.8
DQ822611 HwSSA4 Heat shock protein 70 14. 16. 32. 1.8
AY731090 HwSTT3 Catalytic subunit of the oligosaccharyltransferase complex 1. 14. 2.8
DQ822640 HwSUN4 Cell wall synthesis protein related to glucanases 1. 40. 42. -2.5
DQ822676 HwTDH1 Glyceraldehyde-3-phosphate dehydrogenase 1. 2. 8.9
DQ858159 HwTEF1 Eukaryotic elongation factor 1-alpha (EF-1A) 12. 2.1
DQ822658 HwTIF1 Ekcaryotic translation initiation factor 1-alpha (eIF-4A) 12. 5.0
DQ822609 HwTKL1 Transketolase 1. 2. 5.2
DQ822632 HwTOM40 Mitochondrial import receptor translocase 14. 20. 4.0
DQ822644 HwTPM1 Tropomyosin 16. 20. 34. 40. 42. 43. ND
DQ822647 HwTUB1 Alpha-tubulin 10. 14. 42. ND
DQ822648 HwTUB2 Beta-tubulin 10. 14. 42. -1.8
DQ822653 HwUGP1 UDP-glucose pyrophosphorylase 1. 2. 14. 2.8
DQ822637 HwVPS34 Phosphatidylinositol 3-kinase homolog 1. 14. 20. 30. ND
DQ858157 SOL10 Weakly similar to H. sapiens cyclin-dependent kinase 6 ND ND
DQ822616 SOL11 Mannose-P-dolichol utilization defect 1 protein (14. 16. 20.) -1.5
DQ822618 SOL12 Ubiquitin associated protein 2-like protein (14. 16. 32.) ND
DQ822636 SOL13 Opsin 1 (2. 20.) 2.1
DQ822638 SOL14 Histidine-rich glycoprotein precursor (32. 40.) ND
DQ822639 SOL15 Acetyl xylan esterase (1. 32. 34.) 2.2
DQ822650 SOL16 Senescence-associated protein (10. 11. 40.) 1.8
DQ822652 SOL17 Feruloyl esterase (1. 32. 34.) 2.0
DQ822674 SOL18 Hyperosmolarity-induced mRNA 18 ND 6.9
DQ822675 SOL19 Weakly similar to yeast stress response transcription factor Crz1 ND -1.9
DQ822676 SOL20 Weakly similar to yeast nitrosoguanidine resistance protein Sng1 ND 2.4
DQ822677 SOL21 Weakly similar to yeast prespliceosomal RNA helicase Prp5 ND 7.2
DQ822678 SOL22 Hyperosmolarity-induced mRNA 22 ND ND
DQ822679 SOL23 Weakly similar to yeast Hsp70 protein Ssz1 ND 5.5
DQ822680 SOL24 Weakly similar to yeast t-SNARE protein Sso1 ND 3.8
DQ822681 SOL25 Hyperosmolarity-induced mRNA 25 ND 5.5
DQ822682 SOL26 Weakly similar to yeast transcriptional repressor Rgm1 ND 6.7
DQ858160 SOL27 Hyperosmolarity-induced mRNA 27 ND 6.3
DQ822683 SOL28 Hyperosmolarity-induced mRNA 28 ND 5.0
DQ858162 SOL29 Weakly similar to yeast histone deacetylase complex subunit Pho23 ND ND