Table 1 Differentially expressed genes in H. werneckii adaptation to 3 M NaCl or 4.5 M NaCl. BLASTX matches of EST clones derived from the Hw4.5-3 subtraction library and their functional categorizations based on MIPS are collected. Similarities with probability <10-5 were regarded as being significant, and others as not determined (ND). Genes selected for the expression and chromatin immunoprecipitation analyses are printed in bold. aGenBank accession number. bNumbers indicate the functional groups (see Table 3). Numbers in brackets correspond to literature-based arbitrarily-assigned functional categories for ESTs with orthologs in organisms other than S. cerevisiae. cFold induction (fI, positive value) or fold repression (fR, negative value) in 4.5 M NaCl- vs. 3 M NaCl-adapted H. werneckii cells. Numbers indicated are mean values of three independent RT-PCR experiments, and the representative gels are shown in figures 1 and 2.
GenBank.a | Gene | Characteristic or description | Functional categoryb | fI/f Rc |
|---|---|---|---|---|
DQ822684 | HwAGP1 | Amino acid permease | 20. | 3.3 |
DQ822624 | HwATP1 | Mitochondrial ATPase alpha-subunit | 2. 20. 34. | 1.8 |
DQ822625 | HwATP2 | Mitochondrial ATPase beta-subunit | 2. 20. 34. 40. | 4.0 |
DQ822626 | HwATP3 | Mitochondrial ATPase gamma-subunit | 2. 20. 34. | 2.3 |
DQ822606 | HwBMH1 | 14-3-3 protein | 1. 10. 16. 30. 40. 43. | 1.8 |
DQ822642 | HwCDC3 | Septin complex component | 10. 16. 34. 40. 42. 43. | -3.3 |
DQ822620 | HwCIT1 | Mitochondrial citrate synthase | 1. 2. 42. | 4.6 |
DQ822622 | HwCOB1 | Cytochrome b | 2. | 5.2 |
DQ822621 | HwCOX1 | Cytochrome c oxidase subunit I | 2. 20. | 2.5 |
DQ822687 | HwCYT1 | Cytochrome c1 | 2. 20. | 3.6 |
DQ822643 | HwDBP2 | RNA helicase | 1. 11. | -4.2 |
DQ822641 | HwECM33 | Extracellular matrix 33 protein | 10. 42. 43. | 2.0 |
DQ822657 | HwEFT2 | Eukaryotic translation elongation factor 2 (eEF-2) | 12. | 1.9 |
DQ822685 | HwELF1 | Transcription elongation factor | 10. 11. 40 | 7.6 |
DQ822613 | HwERV25 | p24 component of the COPII-coated vesicles | 20. | 9.4 |
DQ822619 | HwFAS1 | Mitochondrial acyl-carrier protein | 1. | 3.1 |
DQ822689 | HwFRE7 | Ferric-chelate reductase-7 transmembrane component | 20. 34. | 10.5 |
DQ822656 | HwFUN12 | Eukaryotic translation initiation factor 5B (eIF-5B) | 12. | 3.4 |
DQ822655 | HwGAL10 | UDP-glucose 4-epimerase | 1. | ND |
DQ822610 | HwGDH1 | NADP(+)-specific glutamate dehydrogenase | 1. 2. 16. | 7.5 |
DQ822654 | HwGND2 | 6-phosphogluconate dehydrogenase | 1. 2. 16. | 4.1 |
DQ822631 | HwGUT2 | FAD-dependent glycerol-3-phosphate dehydrogenase | 1. 2. 42. | 3.0 |
DQ822645 | HwHHF1 | Histone H4 | 10. 11. 16. | ND |
DQ822614 | HwHSP82 | Heat shock protein 90 | 1. 2. 10. 14. 32. 34. 43. | 5.1 |
DQ822646 | HwHTA1 | Histone H2A | 10. 11. 16. | -2.6 |
DQ858164 | HwIRE1 | Serine-threonine protein kinase and endoribonuclease | 1. 11. 14. 30. 32. | 2.1 |
DQ822615 | HwKAR2 | Endoplasmic reticulum luminal chaperone | 1. 10. 14. 16. 20. 32. 41. 42. | 2.3 |
DQ822627 | HwKGD2 | Dihydrolipoamide succinyltransferase | 1. 2. | 1.8 |
DQ822629 | HwLSC2 | Mitochondrial succinyl-CoA ligase beta-chain | 1. 2. 16. | 2.7 |
DQ822630 | HwMDH1 | Mitochondrial malate dehydrogenase | 1. 2. 42. | 3.4 |
DQ858163 | HwMET14 | ATP adenosine-5'-phosphosulfate 3'-phosphotransferase | 1. | 3.4 |
DQ822607 | HwMET17 | Cystein synthase | 1. | 8.2 |
DQ822605 | HwMET6 | Cobalamin-independent methionine synthase | 1. | 3.2 |
DQ822628 | HwMIR1 | Mitochondrial phosphate transport protein | 20. 34. | 8.4 |
DQ822649 | HwNHP6A | High mobility group protein A | 10. 11. 40. 43. | 4.5 |
DQ822623 | HwNUC1 | Mitochondrial nuclease | 1. 10. | 3.2 |
DQ822635 | HwOPI3 | Unsaturated phospholipid methyltransferase | 1. | 9.7 |
DQ822612 | HwPDI1 | Protein disulphide isomerase precursor | 14. 20. | 2.1 |
DQ858158 | HwPGK1 | 3-phosphoglycerate kinase | 1. 2. 16. | 6.4 |
DQ822634 | HwPMA2 | Plasma membrane proton-exporting ATPase | 2. 20. 34. | 7.9 |
DQ822651 | HwPRY1 | Pathogenesis-related protein precursor | 34. | 7.5 |
DQ822686 | HwPUF1 | Serine rich pumilio family RNA binding domain protein | 1. 10. 11. 16. | 4.6 |
DQ822688 | HwRAD16 | SWI/SNF family DEAD/DEAH box helicase | 10. 32. | 4.9 |
DQ822660 | HwRPL10 | 60S ribosomal protein 10 | 10. 12. 14. | ND |
DQ822672 | HwRPL16A | 60S ribosomal protein 16A | 12. 16. | ND |
DQ822664 | HwRPL22A | 60S ribosomal protein 22A | 12. | ND |
DQ822670 | HwRPL2B | 60S ribosomal protein 2B | 12. | ND |
DQ822671 | HwRPL3 | 60S ribosomal protein 3 | 12. | ND |
DQ822669 | HwRPL6A | 60S ribosomal protein 6A | 12. 16. | 2.6 |
DQ822661 | HwRPL7B | 60S ribosomal protein 7B | 12. | ND |
DQ822617 | HwRPN2 | 26S proteasome regulatory particle subunit | 11. 40. 30. | 2.2 |
DQ822673 | HwRPS10B | 40S ribosomal protein 10B | 12. | ND |
DQ822666 | HwRPS12 | 40S ribosomal protein 12 | 12. | ND |
DQ822665 | HwRPS15 | 40S ribosomal protein 15 | 12. 14. 20. | ND |
DQ822667 | HwRPS16A | 40S ribosomal protein 16A | 12. | 7.6 |
DQ822663 | HwRPS17A | 40S ribosomal protein 17A | 12. | ND |
DQ822668 | HwRPS26B | 40S ribosomal protein 26B | 12. | ND |
DQ822659 | HwRPS8A | 40S ribosomal protein 8A | 12. | ND |
DQ822662 | HwRPS8B | 40S ribosomal protein 8B | 12. | ND |
DQ822608 | HwSAM2 | S-adenosylmethionine synthetase | 1. | 2.8 |
DQ822633 | HwSHY1 | Mitochondrial inner membrane protein chaperone | 2. 16. 32. | 2.5 |
DQ858161 | HwSKN1 | Beta-1,6-glucan synthetase | 1. 2. 42. | 2.8 |
DQ822611 | HwSSA4 | Heat shock protein 70 | 14. 16. 32. | 1.8 |
AY731090 | HwSTT3 | Catalytic subunit of the oligosaccharyltransferase complex | 1. 14. | 2.8 |
DQ822640 | HwSUN4 | Cell wall synthesis protein related to glucanases | 1. 40. 42. | -2.5 |
DQ822676 | HwTDH1 | Glyceraldehyde-3-phosphate dehydrogenase | 1. 2. | 8.9 |
DQ858159 | HwTEF1 | Eukaryotic elongation factor 1-alpha (EF-1A) | 12. | 2.1 |
DQ822658 | HwTIF1 | Ekcaryotic translation initiation factor 1-alpha (eIF-4A) | 12. | 5.0 |
DQ822609 | HwTKL1 | Transketolase | 1. 2. | 5.2 |
DQ822632 | HwTOM40 | Mitochondrial import receptor translocase | 14. 20. | 4.0 |
DQ822644 | HwTPM1 | Tropomyosin | 16. 20. 34. 40. 42. 43. | ND |
DQ822647 | HwTUB1 | Alpha-tubulin | 10. 14. 42. | ND |
DQ822648 | HwTUB2 | Beta-tubulin | 10. 14. 42. | -1.8 |
DQ822653 | HwUGP1 | UDP-glucose pyrophosphorylase | 1. 2. 14. | 2.8 |
DQ822637 | HwVPS34 | Phosphatidylinositol 3-kinase homolog | 1. 14. 20. 30. | ND |
DQ858157 | SOL10 | Weakly similar to H. sapiens cyclin-dependent kinase 6 | ND | ND |
DQ822616 | SOL11 | Mannose-P-dolichol utilization defect 1 protein | (14. 16. 20.) | -1.5 |
DQ822618 | SOL12 | Ubiquitin associated protein 2-like protein | (14. 16. 32.) | ND |
DQ822636 | SOL13 | Opsin 1 | (2. 20.) | 2.1 |
DQ822638 | SOL14 | Histidine-rich glycoprotein precursor | (32. 40.) | ND |
DQ822639 | SOL15 | Acetyl xylan esterase | (1. 32. 34.) | 2.2 |
DQ822650 | SOL16 | Senescence-associated protein | (10. 11. 40.) | 1.8 |
DQ822652 | SOL17 | Feruloyl esterase | (1. 32. 34.) | 2.0 |
DQ822674 | SOL18 | Hyperosmolarity-induced mRNA 18 | ND | 6.9 |
DQ822675 | SOL19 | Weakly similar to yeast stress response transcription factor Crz1 | ND | -1.9 |
DQ822676 | SOL20 | Weakly similar to yeast nitrosoguanidine resistance protein Sng1 | ND | 2.4 |
DQ822677 | SOL21 | Weakly similar to yeast prespliceosomal RNA helicase Prp5 | ND | 7.2 |
DQ822678 | SOL22 | Hyperosmolarity-induced mRNA 22 | ND | ND |
DQ822679 | SOL23 | Weakly similar to yeast Hsp70 protein Ssz1 | ND | 5.5 |
DQ822680 | SOL24 | Weakly similar to yeast t-SNARE protein Sso1 | ND | 3.8 |
DQ822681 | SOL25 | Hyperosmolarity-induced mRNA 25 | ND | 5.5 |
DQ822682 | SOL26 | Weakly similar to yeast transcriptional repressor Rgm1 | ND | 6.7 |
DQ858160 | SOL27 | Hyperosmolarity-induced mRNA 27 | ND | 6.3 |
DQ822683 | SOL28 | Hyperosmolarity-induced mRNA 28 | ND | 5.0 |
DQ858162 | SOL29 | Weakly similar to yeast histone deacetylase complex subunit Pho23 | ND | ND |