Figure 3

Comparison of northern blot results with microarrays data. A) RNA samples from each of the fractions collected for microarray analysis were separated on formaldehyde-agarose gel and subjected to northern blotting. Three blots were prepared, one from each experimental repeat (indicated by bars at the left). Blots were hybridized with probes complementary to the genes indicated at left of each panel and with a probe for the spiked-in Phe RNA. B) Comparison of quantitation results of the northern analysis with microarray data. Black bars represent the northern analysis signal from each fraction normalized to its corresponding signal of the Phe RNA and calculated as a percent of total signal of that mRNA in the gradient. Open bars represent the ratio obtained in the microarray analysis (note that the histogram has two Y-axes) normalized by a signal from the in vitro transcribed mRNAs. Fractions where open bars are missing indicate spots that did not pass the quality criteria in the microarray assay. Note that the Y-axis scale of the microarray results differs from gene to gene. This is probably due to differences in their mRNAs abundances compared to the reference sample.