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Table 3 Various features of groups of affected mRNAs

From: Genome-wide polysomal analysis of a yeast strain with mutated ribosomal protein S9

Ribosome Differencea Number of Genes CAI ΔG 5UTR (kcal/mol) GC content (%) ΔG ORF (kcal/mol) ORF length (nts) WT abundance (copies/cell) Mutant abundancec (copies/cell) t1/2 (min)
(-4) ≥ 24 0.15b 14.8 39 285 1189 1.7b 1.5 16
(-2)–(-3) 192 0.26 12.8 41 268 1021 4.5 3.6 17.7
(-1) 120 0.25 13.5 42 219 851 5 4.4 16.9
0 307 0.3 12.7 41 327 1354 5.9 4.9 16.5
1 59 0.29 13.2 41 193 766 4.9 3.8 17.1
2–3 87 0.28 14.2 41 198 800 5 4.7 16.2
4 ≤ 26 0.24 13.4 40 565b 2077b 2.8 2.6 18.9
All 815 genes   0.28 13.1 41 281 1075 5.1 4.2 16.9
  1. a – Number of ribosomes in the parental strain (19) minus number in rps9-Δ188 strain (this study).
  2. b – Values that differ significantly from the rest of the genes.
  3. c – The abundance in the mutant was calculated from the transcriptome analysis by multiplying the relative expression in the mutant by values obtained from the wild-type strain (25).