Genome-wide polysomal analysis of a yeast strain with mutated ribosomal protein S9

BMC Genomics

Table 3 Various features of groups of affected mRNAs

Ribosome Difference^a	Number of Genes	CAI	ΔG 5UTR (kcal/mol)	GC content (%)	ΔG ORF (kcal/mol)	ORF length (nts)	WT abundance (copies/cell)	Mutant abundance^c (copies/cell)	t_1/2 (min)
(-4) ≥	24	0.15^b	14.8	39	285	1189	1.7^b	1.5	16
(-2)–(-3)	192	0.26	12.8	41	268	1021	4.5	3.6	17.7
(-1)	120	0.25	13.5	42	219	851	5	4.4	16.9
0	307	0.3	12.7	41	327	1354	5.9	4.9	16.5
1	59	0.29	13.2	41	193	766	4.9	3.8	17.1
2–3	87	0.28	14.2	41	198	800	5	4.7	16.2
4 ≤	26	0.24	13.4	40	565^b	2077^b	2.8	2.6	18.9
All 815 genes		0.28	13.1	41	281	1075	5.1	4.2	16.9

a – Number of ribosomes in the parental strain (19) minus number in rps9-Δ188 strain (this study).
b – Values that differ significantly from the rest of the genes.
c – The abundance in the mutant was calculated from the transcriptome analysis by multiplying the relative expression in the mutant by values obtained from the wild-type strain (25).

ISSN: 1471-2164