A rapid genome-wide response to Drosophila melanogaster social interactions

BMC Genomics

Table 3 Real-time PCR validation of microarray results

Gene identifier	Gene name	Array fold change	Array RT-PCR	Independent RT-PCR
CG1732		1.89	2.94	2.86
CG17820	fit	1.6	2.48	1.25
CG7592	Obp99b	1.52	1.51	1.35
CG7106	lectin-28C	1.48	1.2	1.92
CG16834	lectin-33a	1.87	5.37	4.91
CG4740	AttC	-11.79	-19.26	-3.01
CG4757		-7.69	-3.73	1.07
CG15066	IM23	-5.17	-6.46	-1.07
CG8175	Mtk	-4.37	-2.41	1.01
CG1367	CecA2	-4.31	-3.75	-1.04
CG13422		-4	-5.25	2.11
CG10816	Dro	-3.3	-40.05	-1.33
CG6687		-2.84	-2.82	1.07
CG9434	Fst	-1.76	-2.79	1.6

Five genes up regulated in courting males were verified by Real-time PCR on the samples used for array hybridization (Array RT-PCR) as well as by analysis of independently obtained samples (Independent RT-PCR). Nine down-regulated genes were validated using array samples, while 4 of 9 were validated on independent samples.

ISSN: 1471-2164