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Table 3 Real-time PCR validation of microarray results

From: A rapid genome-wide response to Drosophila melanogaster social interactions

Gene identifier Gene name Array fold change Array RT-PCR Independent RT-PCR
CG1732   1.89 2.94 2.86
CG17820 fit 1.6 2.48 1.25
CG7592 Obp99b 1.52 1.51 1.35
CG7106 lectin-28C 1.48 1.2 1.92
CG16834 lectin-33a 1.87 5.37 4.91
CG4740 AttC -11.79 -19.26 -3.01
CG4757   -7.69 -3.73 1.07
CG15066 IM23 -5.17 -6.46 -1.07
CG8175 Mtk -4.37 -2.41 1.01
CG1367 CecA2 -4.31 -3.75 -1.04
CG13422   -4 -5.25 2.11
CG10816 Dro -3.3 -40.05 -1.33
CG6687   -2.84 -2.82 1.07
CG9434 Fst -1.76 -2.79 1.6
  1. Five genes up regulated in courting males were verified by Real-time PCR on the samples used for array hybridization (Array RT-PCR) as well as by analysis of independently obtained samples (Independent RT-PCR). Nine down-regulated genes were validated using array samples, while 4 of 9 were validated on independent samples.