Figure 2

Specificity of miRNA microarray hybridization. The control oligos in the 3 DNA array900 microRNA direct kit were ligated with Cy5 capture sequence and hybridized onto one block. The mismatched probes had one (m1), two (m2) or three (m3) mismatched nucleotides with the corresponding control oligos. The signals from the slide were processed with the method described in Materials and Methods. Data shown were means ± S.E. from 3 hybridizations.