Figure 4
Northern blot validation. 15 μg total RNA was separated on a denaturing 15% PAGE gel. The 32P labeled probes for the microRNA were hybridized to the membrane overnight. The U6 snRNA was probed as the loading control after the hybridization of microRNA probes. (a) lung-specific miRNAs; (b) kidney-specific miRNAs; (c) co-expressed miRNAs in the lung and heart identified by HSD; (d) miRNAs highly expressed in the lung and/or the heart. (e) commonly expressed miRNAs.