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Table 5 Microarray platforms differentially discriminate the various actin isoforms

From: Design and evaluation of Actichip, a thematic microarray for the study of the actin cytoskeleton

Actin isoforms Actichip Affymetrix Operon
  MCF-7 Skeletal muscle MCF-7 Skeletal muscle MCF-7 Skeletal muscle
Actin, alpha 1, skeletal muscle (ACTA1) - + - + + +
Actin, alpha 2, smooth muscle, aorta (ACTA2) - - + + - +
Actin, beta (ACTB) + + + + + +
Actin, alpha, cardiac muscle 1 (ACTC1) - + - + Ø Ø
Actin, gamma 1 (ACTG1) + + + + - -
Actin, gamma 2, smooth muscle, enteric (ACTG2) - - + + - -
  1. Hybridisation data were treated following the procedure specific for each platform (see "Methods") to identify genes reproducibly deemed expressed (+) or not expressed (-). The table summarises the expression data obtained for the different actin isoforms. Ø : Isoform not represented on the array.