Design and evaluation of Actichip, a thematic microarray for the study of the actin cytoskeleton

BMC Genomics

Table 5 Microarray platforms differentially discriminate the various actin isoforms

Actin isoforms	Actichip		Affymetrix		Operon
	MCF-7	Skeletal muscle	MCF-7	Skeletal muscle	MCF-7	Skeletal muscle
Actin, alpha 1, skeletal muscle (ACTA1)	-	+	-	+	+	+
Actin, alpha 2, smooth muscle, aorta (ACTA2)	-	-	+	+	-	+
Actin, beta (ACTB)	+	+	+	+	+	+
Actin, alpha, cardiac muscle 1 (ACTC1)	-	+	-	+	Ø	Ø
Actin, gamma 1 (ACTG1)	+	+	+	+	-	-
Actin, gamma 2, smooth muscle, enteric (ACTG2)	-	-	+	+	-	-

Hybridisation data were treated following the procedure specific for each platform (see "Methods") to identify genes reproducibly deemed expressed (+) or not expressed (-). The table summarises the expression data obtained for the different actin isoforms. Ø : Isoform not represented on the array.

ISSN: 1471-2164