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Figure 5 | BMC Genomics

Figure 5

From: Revised genomic structure of the human ghrelin gene and identification of novel exons, alternative splice variants and natural antisense transcripts

Figure 5

Exon structure of exon -1 to 4 RT-PCR amplicons in human tissues and continuous cell lines. Exons are represented as boxes, PCR primers are indicated as arrows above exons. Transcripts have been grouped into three major types (I, II and III). The tissue where an amplicon was first sequenced from is depicted on the left hand side of each transcript's exon structure. Group I are preproghrelin mRNA variants with different 5' untranslated regions (5' UTRs). Group II lack the coding region 'active core' of ghrelin (in exon 1) and contains one or more downstream exons (2–4). Group III contains the novel exons -1*, 4*, 2* and 2** in various combinations. PBL denotes leukocytes; PC3 is a human prostate carcinoma cell line. Group I: 855 bp, stomach [GenBank:EF549571];777 bp, stomach [GenBank:EF549572];580 bp, stomach [GenBank:EF549573];852 bp, heart [GenBank:EU072085];1316 bp, placenta [GenBank:EU072083];1313 bp, kidney [GenBank:EU072084];1067 bp, kidney [GenBank:EU072087]. Group II: 443 bp, stomach [GenBank:EF549574];326 bp, stomach [GenBank:EF549574];440 bp, PBL [GenBank:EU072086];217 bp, PC3 [GenBank:EF549557]. Group III: 1176 bp, stomach [GenBank:EF549568];921 bp, stomach [GenBank:EF549570];1108 bp, spleen [GenBank:EU072081];950 bp, kidney [GenBank:EU072082].

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