Table 2 Clusters of combined P. papatasi midgut cDNA libraries (sugar-fed, blood-fed and Leishmania major -infected) of transcripts with high quality sequences
Cluster | Clone | NCBI best match to NR database | E value | Putative function | GenBank |
|---|---|---|---|---|---|
Cluster 1 | B05_ppmgbl_p23 | microvilli membrane protein [A. aegypti ] | 1.0E-47 | Microvilli protein | EU031911 |
Cluster 2 | F05_ppmgbl_p23 | microvilli membrane protein [A. aegypti ] | 1.0E-47 | Microvilli protein | EU031911 |
Cluster 3 | PPMGBM189 | microvilli membrane protein [A. aegypti] | 7.0E-48 | Microvilli protein | EU031911 |
Cluster 9 | PPMGBL17 | LP07759p [D. melanogaster ] | 8.0E-25 | Peritrophin | EU031912 |
Cluster 10 | A05_PPMGBS_P28 | hypothetical protein 17 [L. obliqua ] | 3.0E-44 | 40S ribosomal S30 protein | EU040041 |
Cluster 11 | A07_PPMGBS_P28 | ENSANGP00000028746 [A. gambiae ] | 1.0E-33 | Unknown | EU040042 |
Cluster 12 | PPMGM173 | similar to CG4778-PA [T. castaneum ] | 8.0E-11 | Peritrophin | EU047543 |
Cluster 13 | A02_PPINFL_P30 | similar to CG4778-PA [T. castaneum ] | 7.0E-11 | Peritrophin | EU047543 |
Cluster 15 | H08_PPMGBL_P31 | chymotrypsin [P. papatasi] | 1.0E-154 | Chymotrypsin | AAM96939 |
Cluster 16 | E10_ppmgbm_p21 | carboxypeptidase B [A. aegypti ] | 4.0E-67 | Carboxypeptidase | EU047544 |
Cluster 17 | C07_PPMGS_P24 | ribosomal protein S20 [B. mori ] | 9.0E-57 | 40S ribosomal protein S20 | EU047545 |
Cluster 18 | H10_ppmgm_p22 | trypsin 1 [P. papatasi ] | 1.0E-151 | Trypsin | AAM96940 |
Cluster 20 | H05_ppmgm_p22 | CG32276-PB, isoform B [D. melanogaster ] | 2.0E-23 | Ribosome associated membrane protein | EU047546 |
Cluster 21 | PPMGBS47 | Ribosomal protein L19 [D. melanogaster ] | 1.0E-100 | 60s ribosomal protein L19 | EU047547 |
Cluster 23 | D05_PPMGL_P28 | trypsin 2 [P. papatasi ] | 1.0E-157 | Trypsin | AAM96941 |
Cluster 24 | G04_PPMGM_P25 | RE59709p [D. melanogaster ] | 7.0E-68 | 60S ribosomal protein L32 | EU047548 |
Cluster 25 | PPMGS_P31_B06 | similar to D. melanogaster CG3203 [D. yakuba ] | 1.0E-91 | 60S ribosomal protein L17 | EU045355 |
Cluster 26 | PPMGL_P29_D06 | peritrophin-like protein 1 [C. felis ] | 2.0E-36 | Peritrophin | EU045354 |
Cluster 29 | PPINFM-P7-G10 | Ribosomal protein L29 [D. melanogaster ] | 4.0E-24 | 60S ribosomal protein L29 | EU045353 |
Cluster 31 | B03_PPMGM_P25 | CG13551 [D. melanogaster ] | 3.0E-37 | Unknown | EU049582 |
Cluster 32 | H04_PPMGL_P23 | LD17235p [D. melanogaster ] | 1.0E-93 | 60S ribosomal protein L11 | EU045352 |
Cluster 34 | PPMGM152 | similar to D. melanogaster RpL14 [D. yakuba ] | 5.0E-50 | 60S ribosomal protein L14 | EU045351 |
Cluster 35 | E07_ppmgs_p21 | 60S acidic ribosomal protein P1 [S. frugiperda ] | 1.0E-45 | 60s Acidic ribosomal protein P1 | EU045350 |
Cluster 37 | PPMGL197 | ENSANGP00000019623 [A. gambiae ] | 4.0E-63 | Astacin | EU045349 |
Cluster 40 | F01_PPINFL_P30 | S7 ribosomal protein [C. pipiens quinquefasciatus ] | 3.0E-89 | 40S ribosomal protein S7 | EU045348 |
Cluster 73 | A03_PPPMGBM_P25 | unknown [C. sonorensis ] | 4.0E-25 | Unknown | EU045347 |
Cluster 75 | C04_ppmgs_p21 | similar to D. melanogaster qm [D. yakuba ] | 1.0E-117 | 60s ribosomal protein L10 | EU045346 |
Cluster 89 | B11_PPINFL_P32 | trypsin 4 [P. papatasi ] | 1.0E-129 | Trypsin | AAM96943 |
Cluster 94 | F08_PPINFM_P22 | microvilli membrane protein [A. aegypti ] | 2.0E-35 | Microvilli protein | EU047549 |
Cluster 96 | C05_ppmgm_p22 | Cr-PII [P. americana ] | 3.0E-20 | Microvilli protein | EU047550 |
Cluster 98 | B12_ppmgbl_p23 | ENSANGP00000017713 [A. gambiae ] | 1.0E-17 | Microvilli protein | EU047551 |
Cluster 99 | A03_ppmgbl_p20 | hypothetical protein [T. castaneum ] | 2.0E-23 | Unknown | EU045345 |
Cluster 103 | H03_PPMGL_P23 | GA13179-PA [D. pseudoobscura ] | 2.0E-43 | Ferritin | EU045344 |
Cluster 106 | H07_ppmgm_p22 | TPA_inf: HDC07203 [D. melanogaster ] | 8.0E-14 | Unknown | EU045343 |
Cluster 111 | PPMGL_P34_H09 | GA16408-PA [D. pseudoobscura ] | 2.0E-10 | Kazal type serine protease inhibitor | EU045342 |
Cluster 113 | B10_PPINFL_P21 | carboxypeptidase A [A. aegypti ] | 2.0E-87 | Carboxypeptidase | EU045341 |
Cluster 119 | PPMGL_P29_B04 | midgut specific galectin [P. papatasi ] | 1.0E-145 | Galectin | AAT11557 |
Cluster 122 | C08_PPINFL_P31 | GA15307-PA [D. pseudoobscura ] | 3.0E-62 | Ferritin | EU045340 |
Cluster 125 | D06_PPMGL_P23 | Glutathione S-transferase [M. domestica] | 3.0E-86 | Glutathione S-transferase | EU045339 |
Cluster 126 | PPMGBL175 | 10 kDa salivary protein [P. ariasi ] | 6.0E-07 | Unknown | EU045338 |
Cluster 127 | H07_ppmgs_p21 | ribosomal protein S8 [A. albopictus ] | 3.0E-94 | 40S ribosomal protein S8 | EU045337 |
Cluster 128 | C02_ppmgs_p21 | 60S acidic ribosomal protein P2 [A. aegypti ] | 8.0E-39 | 60S acidic ribosomal protein P2 | EU045336 |
Cluster 129 | PPMGL_P29_C01 | ENSANGP00000016569 [A. gambiae ] | 5.0E-40 | membrane LPS inducible TNF protein | EU035828 |
Cluster 134 | C01_PPINFM_P22 | similar to D. melanogaster RpS18 [D. yakuba ] | 9.0E-70 | 40S ribosomal protein S18 | EU035827 |
Cluster 135 | D04_PPINFL_P31 | trypsin 3 [P. papatasi ] | 1.0E-144 | Trypsin | AAM96942 |
Cluster 139 | PPMGS_P31_A11 | CG30415-PB, isoform B [D. melanogaster ] | 1.0E-27 | Unkown | EU035823 |
Cluster 146 | PPMGM132 | similar to CG2998-PA [T. castaneum ] | 2.0E-27 | 40S ribosomal protein S28 | EU035822 |
Cluster 147 | A06_PPMGM_P25 | Ribosomal protein L23 [D. melanogaster ] | 5.0E-73 | 60S ribosomal protein L23 | EU035821 |
Cluster 149 | B06_PPMGM_P25 | similar to D. melanogaster RpS12 [D. yakuba ] | 1.0E-64 | 40S ribosomal protein S12 | EU035820 |
Cluster 150 | PPMGL_P29_D08 | ENSANGP00000021011 [A. gambiae ] | 1.0E-111 | Unkown | EU035819 |
Cluster 153 | B07_PPMGS_P24 | similar to D. melanogaster CG2033 [D. yakuba ] | 3.0E-67 | 40S ribosomal protein S15 | EU035818 |
Cluster 158 | D06_PPINFL_P21 | ENSANGP00000013724 [A. gambiae ] | 5.0E-70 | Ryanodine receptor | EU035817 |
Cluster 163 | PPMGM133 | cyclophylin isoform [A. aegypti ] | 8.0E-82 | Cyclophilin | EU032351 |
Cluster 165 | A11_PPMGM_P25 | 60S ribosomal protein L40 [A. albopictus ] | 4.0E-68 | Ubiquitin/ribosomal L40 fusion | EU032350 |
Cluster 167 | PPMGL276 | similar to ENSANGP00000002356 [A. mellifera ] | 2.0E-66 | Na+/K+ ATPase | EU032348 |
Cluster 168 | F05_ppmgbm_p21 | chymotrypsin [P. papatasi ] | 1.0E-147 | Chymotrypsin | AAM96938 |
Cluster 171 | G12_ppmgs_p21 | GA16582-PA [D. pseudoobscura ] | 8.0E-78 | 60S ribosomal protein L12 | EU032349 |
Cluster 174 | A06_ppmgm_p22 | similar to D. melanogaster CG2099 [D. yakuba ] | 1.0E-54 | 60S ribosomal protein L35A | EU032347 |
Cluster 176 | A03_pppmgbl_p24 | translation factor SUI1-like protein [A. aegypti ] | 1.0E-54 | Translation initiation factor 1 | EU032346 |
Cluster 177 | A08_PPMGBS_P28 | GA10714-PA [D. pseudoobscura ] | 5.0E-92 | ADP ribosylation factor | EU032345 |
Cluster 182 | PPMGS_P31_E04 | similar to D. melanogaster CG10423 [D. yakuba ] | 2.0E-38 | 40s ribosomal protein S27 | EU032344 |
Cluster 183 | D12_ppmgm_p22 | ENSANGP00000026718 [A. gambiae ] | 1.0E-61 | Cytochrome C oxidase subunit IV | EU032343 |
Cluster 184 | C02_PPINFL_P30 | unknown [C. sonorensis ] | 4.0E-21 | Unknown | EU032342 |
Cluster 185 | D04_PPINFM_P27 | cytochrome b [P. papatasi ] | 1.0E-110 | Cytochrome B | AF161214 |
Cluster 186 | MGL69 | similar to CG9916-PA isoform 1 [T. castaneum ] | 4.0E-81 | Cyclophilin | EU032341 |
Cluster 187 | PPINFM-P7-F11 | ribosomal protein S17e [Eucinetus sp. ] | 9.0E-31 | 40S ribosomal protein S17 | EU032340 |
Cluster 188 | H01_PPMGL_P23 | 10 kDa salivary protein [P. ariasi ] | 2.0E-06 | Unknown | EU032339 |
Cluster 201 | G11_PPPMBGM_P26 | larval chymotrypsin-like protein [A. aegypti ] | 5.0E-82 | Chymotrypsin | EU035826 |
Cluster 228 | PPMGM508 | peroxiredoxin-like protein [A. aegypti ] | 1.0E-69 | Peroxiredoxin | EU035825 |
Cluster 232 | PPMGBL92 | glutathione S-transferase [A. aegypti ] | 4.0E-59 | Glutathione S-transferase | EU035824 |
Cluster 243 | D11_pppmgbl_p24 | midgut chitinase [P. papatasi ] | 1.0E-140 | chitinase | AAV49322 |