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Figure 2 | BMC Genomics

Figure 2

From: Glycerate kinase of the hyperthermophilic archaeon Thermoproteus tenax: new insights into the phylogenetic distribution and physiological role of members of the three different glycerate kinase classes

Figure 2

Purification of the recombinant class II glycerate kinase of T. tenax. SDS-PAGE of the recombinant expression and purification of T. tenax glycerate kinase. Lanes with crude cell extracts (CE, 20 μg), soluble fractions after heat precipitation (HP, 10 μg) and after purification by Q sepharose (QS), phenyl sepharose (PS) and gel filtration (GF) (5 μg) are shown. 'M' refers to the protein marker, Dalton Mark VII-L (Sigma)).

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