DNA targets | PCR primer code | DNA sequence | position on Bcen-J2315 genome | annealing temperature |
---|---|---|---|---|
ecfA1 region | A | CACGGTCATCACGCTCGC | Chr1 3124945-3124962 | 55°C |
ecfA1 and ecfA2 region | B | TTCGGTGCGATAACCAGC | Chr1 1056358-1056374 | 55°C |
ecfA2 region | C | CGGGCGCAAGAACATACA | Chr1 1056771-1056789 | 55°C |
ecfB | ecfB-F | CGCTTCGCGCTCTGGCTG | Chr1 3817718-3817735 | 58°C |
ecfB | ecfB-R | TCAGCCGCAAGGAAGGAGC | Chr1 3818171-3818153 | 58°C |
ecfD | ecfD-F | CGCAACTGCGGCGCCCG | Chr1 2115044-2115060 | 55°C |
ecfD | ecfD-R | CGAGCGTTTCGAGCGGGTC | Chr1 2615449-2615467 | 55°C |
ecfE | ecfE-F | GTGAACGAACGAGGCTAGG | Chr1 2728191-2728210 | 55°C |
ecfE | ecfE-R | CTCCTTGAAGGACTGTCC | Chr1 2727807-2727824 | 55°C |
sigI | sigI-F | ATCCCGCTCGATGCCGCGC | Chr2 2667864-2667882 | 55°C |
sigI | sigI-R | GAGAATTTGAAGCCGCGCCG | Chr2 2668242-2668261 | 55°C |
sigI | sigIv-F | ATCATGCCCGCTGGCTC | Chr3* 1181202-1181218 | 55°C |
sigI | sigIv-R | AACAGCCCGATGTTGCCT | Chr3* 1181508-1181491 | 55°C |
sigJ | sigJ-F | CATGCGCATGGCACCCGTTC | Chr2 1383013-1383032 | 55°C |
sigJ | sigJ-R | GAGAATTTGAAGCCGCGCCG | Chr2 2668242-2668261 | 55°C |