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Table 3 PCR primers used in this study.

From: Architecture of Burkholderia cepacia complex σ70 gene family: evidence of alternative primary and clade-specific factors, and genomic instability

DNA targets

PCR primer code

DNA sequence

position on Bcen-J2315 genome

annealing temperature

ecfA1 region

A

CACGGTCATCACGCTCGC

Chr1 3124945-3124962

55°C

ecfA1 and ecfA2 region

B

TTCGGTGCGATAACCAGC

Chr1 1056358-1056374

55°C

ecfA2 region

C

CGGGCGCAAGAACATACA

Chr1 1056771-1056789

55°C

ecfB

ecfB-F

CGCTTCGCGCTCTGGCTG

Chr1 3817718-3817735

58°C

ecfB

ecfB-R

TCAGCCGCAAGGAAGGAGC

Chr1 3818171-3818153

58°C

ecfD

ecfD-F

CGCAACTGCGGCGCCCG

Chr1 2115044-2115060

55°C

ecfD

ecfD-R

CGAGCGTTTCGAGCGGGTC

Chr1 2615449-2615467

55°C

ecfE

ecfE-F

GTGAACGAACGAGGCTAGG

Chr1 2728191-2728210

55°C

ecfE

ecfE-R

CTCCTTGAAGGACTGTCC

Chr1 2727807-2727824

55°C

sigI

sigI-F

ATCCCGCTCGATGCCGCGC

Chr2 2667864-2667882

55°C

sigI

sigI-R

GAGAATTTGAAGCCGCGCCG

Chr2 2668242-2668261

55°C

sigI

sigIv-F

ATCATGCCCGCTGGCTC

Chr3* 1181202-1181218

55°C

sigI

sigIv-R

AACAGCCCGATGTTGCCT

Chr3* 1181508-1181491

55°C

sigJ

sigJ-F

CATGCGCATGGCACCCGTTC

Chr2 1383013-1383032

55°C

sigJ

sigJ-R

GAGAATTTGAAGCCGCGCCG

Chr2 2668242-2668261

55°C

  1. * Position on B. vietnamiensis G4 genome