| Â | Eco RI-digested library | Bam HI-digested library |
|---|
Vector | pBACe3.6 [52] | pBeloBAC11 [53] |
Cloning site | Eco RI | Bam HI |
Number of clones | 36000 (96 × 384 wells) | 21120 (55 × 384 wells) |
Mean insert size (kbp) | 168 | 165 |
Clone coverage | × 11.4 | × 6.6 |
Strain | p50T (mixed insects) | p50T (mixed insects) |
- To calculate the percentage of the silkworm genome covered by the clones (clone coverage) in the Eco RI- and Bam HI-digested libraries, we assumed that the silkworm genome size was 530 Mb [34]. Detailed information on the Eco RI-digested library, such as the size distribution of BAC inserts, is available in the paper cited [49] and at the RPCI-96 BAC Library website [32]. Detailed information on the Bam HI-digested library can be obtained from the website of Texas A&M BAC Libraries [33].
