Figure 1From: Giant panda BAC library construction and assembly of a 650-kb contig spanning major histocompatibility complex class II region(a) Insert size analysis of randomly selected BAC clones. There are 14 BAC clones tested. The BAC DNA was completely digested with Not I enzyme and determined by pulsed field gel electrophoresis with 1–20 second switch time, 6 V/cm at 12°C for 16 hours. The MidRange II PFG markers (New England BioLabs) were used as size standard in the right flanking lane. The size of pCC1BAC vector is 8.1 kb. (b) Distribution of insert size in the giant panda BAC library. Insert sizes were evaluated by PFGE after Not I restriction enzyme digestion from 174 BAC clones.Back to article page