Table 2 Primer list used for constructing the final contig with minimum tiling path
Primer name | Source | Primer sequences (5'→3') | Size (bp) | GenBank No. |
|---|---|---|---|---|
BTNL2 | GenBank: AY152836.1 GenBank: AJ630362 | GGGAAGATGCTCTACTCA GATGGTCGAATGTTGTTT | 223 | EF125957 |
DRA | GenBank: AY973816 | AGACAAGTTCTCGCCACC CTCCACCTTGCAGTCATAGA | 172 | EF125964 |
DRB | [8] | TTCACCAACGGGACGGAG GCTGCTGCTCCATGAAGTC | 281 | AY895156 |
DQA | [9] | CTTACCCTACTGACCAGC TAAAGAGGCAGAGCATT | 275 | EF664077 |
DOB | GenBank: AY973815 | GGGGACAGTGTATCCAGAG AGGAGTCATTTCCAGCATC | 202 | EF125962 |
LMP2 | GenBank: AY152832.1 GenBank: AJ630364.1 | CCCACTAGAGCCATCCCG CAGCCCTTTGCCATCGGT | 703 | EF125966 |
DMA | GenBank: AY973813 | GAAGCCCCTGGAGTTTGG GGCCTGGAAGCTGAGTTCAT | 157 | EF125960 |
DMB | GenBank: AY973814 | GCTTCTATCCAGCTGATG GGTGTAAGTGTCCCCGTA | 155 | EF125961 |
COL11A2 | GenBank: AY957499.1 GenBank: AJ630366.1 | CTCAAACACTTCTTCATCCAG GACCAGGTGGCACCGTGT | 168 | EF125958 |
DAXX | GenBank: AJ630366.1 GenBank: AY152827.1 GenBank: BC109074.1 | TCGCTCCTGTAACCTGATG CTCTTTGGGCGGCTTTGT | 282 | EF125959 |
237D4-T7 | End sequence | AGTTCTCCATCCGGCTCT TACCATCCTGGTGTCAGT | 172 | / |
237D4-RP2 | End sequence | TTGGAAAGTCTGGGATGA TGTGCTCTGGAAAGATGTG | 274 | / |
826G2-T7 | End sequence | CTCCCTCAGCAATAACC CAAAGAGTGAACCTGCAAAACC | 187 | / |
826G2-RP2 | End sequence | TGTGACCGAGTTGGAGTA GAAAGAGAGATGGGGATG | 442 | / |
900H8-T7 | End sequence | GAAGTGAGGGTAGAGGAC AAGCTGCTGTTAGAAATG | 249 | / |
206G5-T7 | End sequence | AGAGGTCACGACCAGCAC TTTGGAAGGTTGTTTATCAC | 207 | / |
692B2-RP2 | End sequence | AGCACGGCCATAATAATAAA GAGGGACAAGGTTGGACAG | 264 | / |
692B2-F8R8 | Shotgun sequences | TCCTTTAGCCCCTCTTGAGCC CCAACCCGCGTGTACTTTTGG | 195 | / |
1262B6-F5R5 | Shotgun sequences | CCTCCACATTTCTCTGCTT GCCATGGGTATAGTTACCT | 273 | / |
186A7-T7 | End sequence | GCCTCTTGTCCAGTTCCT GGTCTCCCTCTGTTGCTC | 198 | / |