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Table 1 Primer pairs used for characterization

From: Alternative splicing of TGF-betas and their high-affinity receptors TβRI, TβRII and TβRIII (betaglycan) reveal new variants in human prostatic cells

Gene (Acc No)a

Position

Designation

Size

Sequence

ATb

TβRI, human,

256–275c

5-TB1RL

288 bp

GACCACAGACAAAGTTATAC

60°C

(NM_004612)

524–543

3-TB1RL

300 bp

TGGTGAATGACAGTGCGGTT

 

(AJ619019)

159–178

3-HTB1RL

178 bp

TACTGAAAAAGGGCCAGTAG

52°C

TβRII, human

435–454

5-HTBR2B

274 bp

CGCGTATCGCCAGCACGATC

63°C

(NM_003242)

688–708

3-HTBR2B

349 bp

TGGTAGGGGAGCTTGGGGTCA

 

(NM_001024847)

795–815

5-HTBR2E3

298 bp

GTAGCTCTGATGAGTGCAATG

60°C

 

1072–1092

3-HTBR2E4

406 bp

TGGTTGATGTTGTTGGCACAC

 

TβRIIC, human

89–108

5-HTBR2Z

319 bp

GGAGCACTTGTCAAAACACTG

57°C

(AJ786388)

84–115

3-HTBR2CD

115 bp

TCCCAGCCAGTGTTTTGACAAG

60°C

TβRIII, human

2501–2520

5-HTBR3E13

217 bp

TGTGTGCCTCCTGACGAAGC

59°C

(NM_003243)

2609–2717

3-HTBR3E15

 

AGGCTGCAAACGCAATGCCC

 

TGF-β1, human

1402–1420

5-HTGFB1E3

426 bp

TGGCGATACCTCAGCAACC

55°C

(NM_000660)

1809–1827

3-HTGFB1E6

 

GTTGGCATGGTAGCCCTTG

 

TGF-β2, human

680–699

5-HTB2CP

185 bp

CAACAGCACCAGGGACTTGC

65°C

(M19154)

845–864

3-HTB2CP

 

AGCACAAGCTGCCCACTGAG

 

(NM_003238)

658–679

5-TGFB2E1B

272 bp

CCCCGGAGGTGATTTCCATCTA

62°C

 

908–929

3-TGFB2E1B

188 bp

GTAGGGTCTGTAGAAAGTGGGC

 

TGF-β3, human

342–361

5-TGFB3E1

332 bp

TGGACTTCGGCCACATCAAG

57°C

(NM_003239)

653–673

3-TGFB3E2

 

CTCCACTGAGGACACATTGAA

 

GAPDHd, human

402-421

5-GAPDH

300 bp

CGTCTTCACCACCATGGAGA

59°C

(NM_002046)

682-701

3-GAPDH

 

CGGCCATCACGCCACAGTTT

 
  1. aAcc No, EMBL/DDBJ/GenBank Accession Number
  2. bAT, annealing temperature
  3. cthis position is equivalent to 1–20 in AJ619019
  4. dGAPDH, glyceraldehyde-3-phosphate dehydrogenase