Growth phenotypes of pog1Δ cells. (A) Abnormal cellular morphologies of pog1Δ cells. Circled number 1 and 2 indicate dumb-bell cell shapes; number 2 and 3 indicate cells with excessive division septum deposition; number 3 indicates anucleate cells. (B) Subcellular localization of Pog1-γ. Cells bearing a sole copy of pog1+-GFP were examined for GFP localization using fluorescence microscopy. (C) Live cells stained with mitochondria-specific compound MitoTracker. (D) Approximately 5 μl of 10-fold series diluted cells were spotted onto standard EMM medium (glucose) and glycerol medium (glycerol) and incubated at 30°C for 5–7 days. (E) EM images of wild type and pog1Δ cells. Nc and m stand for nucleus and mitochondrion, respectively.