Figure 2

Confirmation of the enrichment of immune-related transcripts in the subtracted cDNA library by quantitative real-time PCR analysis. The relative amount of cDNAs of α-tubulin, Et-AMP10, and eristalin in the subtracted cDNA library (gray bars) is shown relative to their amount in unsubtracted cDNA library (black bars). The cDNA amount of the house-keeping gene α-tubulin was reduced about 18 fold by the subtraction procedure. In contrast, potentially immunity-related genes Et-AMP10 and eristalin were found to be 4 and 38 fold enriched in the subtracted cDNA library, respectively. Results represent mean values of three independent determinations ± S.D.