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Figure 3 | BMC Genomics

Figure 3

From: Analysis of the immune-inducible transcriptome from microbial stress resistant, rat-tailed maggots of the drone fly Eristalis tenax

Figure 3

E. tenax PGRP is homologous to PGRP-SB1 from Drosophila. (A) BlastP search with E. tenax PGRP-SB1 sequence resulted in similar sequences from several Drosophila species and the differences are indicated in the alignment. Red color indicates 90% and blue color 50% consensus. The amino-terminal 24 amino acids represent a putative signal sequence. Accession numbers of PGRP-SB1 proteins are: E. tenax, AM706416; D. simulans, Q70PX8; D. melanogaster, Q70PY2; D. pseudoobscura, Q29DT4. (B) A Bayesian protein tree was generated using the sequence of Eristalis PGRP (Eten PGRP SB1, indicated by a red circle) and sequences of human (Hsap PGRPs), D. melanogaster (Dmel PGRPs), and A. gambiae PGRPs (Agam ENSANGPs) and we found that Ersitalis PGRP grouped nearest to Drosophila PGRP-SB1. T3 lysozyme (N-acetylmuramoyl-L-alanine amidase, P20331) of the bacteriophage T3 was used as out-group. The scale bar represents the substitutions per site according to the model of amino acid evolution applied.

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