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Table 8 Primers used for real time RT-PCR analysis

From: Effect of starvation on global gene expression and proteolysis in rainbow trout (Oncorhynchus mykiss)

Gene name Forward primer Reverse primer GenBank Acc. No./TIGR TC
Capn1 5-GCCAAAACATTGCCTGTTATCTTAG-3 5-ATAGGAGGCCGTATCAAAATTCC-3 AY573919
Capn2 5-GATTCATCCAGAACGTGTAGG-3 5-GGTTAAACACTGGAGCGTGTC-3 AY573920
Cpns 5-GCTGCCTTCAAATCTGCATGT-3 5-TGTACCTGCGAGCGATCAACT-3 AF482696
CAST-S 5-ATGACAGAGCAGCTGTCCAATC-3 5-TGTTGAAGCAACATCACTGCAA-3 AY937408
CAST-L 5-ACGGCACCTTTCCTTTCCATTACCA-3 5-CGGGGGGAGCAGGAGACTTGGT-3 AY937407
Proteasome α5 5-GGTGTAGCGCTTCTCTTTGG-3 5-ACTGGACAAAGGTGCCTGAT-3 TIGR database TC78609
Proteasome β3 5-CCCATGGTGACAGAGGACTT-3 5-TGTCTGGCTCCCAGAGAGAT-3 TIGR database TC87448
Proteasome N3 5-AAGTGAACGACAGCACCATTC-3 5-CCTCATCGATCACCATCTGTT-3 CA386652
Proteasome Regulatory 6 5-CCGACCTCAGAGAAAAGGTG-3 5-AGAAGAGGTACTGGCGGACA-3 TIGR database TC87921
Plyubiqitin 5-CTGGAAGATGGTCGCACTCT-3 5-GATCTGCATACCTCCCCTCA-3 AF361365
Cathepsin-D 5-GCCTGTCATCACATTCAACCT-3 5-CCACTCAGGCAGATGGTCTTA-3 U90321
Cathepsin L 5-TGAAGGAGAAGATGTGGATGG-3 5-TTCCTGTCTTTGGCCATGTAG-3 AF358668
GADPH 5-CTGAACGACCACTTCGTCAA-3 5-TTACTCCTTGGTGGCCATGT-3 CB491826
C3 5-CTAACGAGGGCAAGCTCAAC-3 5-GCCTCCAGAGTGAGAAGGTG-3 CB514355
ATP5J2 5-GGGCGGATAAAAAGGCTAAT-3 5-GCCATTTATTGCCTGAAGGA-3 CB493612
ACP5 5-CAAGCAGTTCGACTGGATCA-3 5-ACCAATGGACCACACAGGAT-3 CB515428
FADS2 5-GTCCGTGCTTTGTGTGAGAA-3 5-TCAGAGACCCGACAACATCA-3 CB494661
β-Actin 5-GCCGGCCGCGACCTCACAGACTAC-3 5-CGGCCGTGGTGGTGAAGCTGTAAC-3 AJ438158