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Figure 1 | BMC Genomics

Figure 1

From: Quantitative measurement of pathogen specific human memory T cell repertoire diversity using a CDR3β-specific microarray

Figure 1

Schematic of overall assay design. Briefly human cDNA is amplified in a non saturating PCR reaction possessing VB17 and Cy-5 labeled JB2.7-specific primers. Purified PCR products are combined with an equimolar mix of synthetic targets complementary to all 108 IRSS-specific CDR3β probes on the array and hybridized, washed and analyzed. Probe design is illustrated at the bottom of the figure.

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BMC Genomics

ISSN: 1471-2164

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